Ex Vivo Thermal Response of Artificially Pigmented Porcine Skin Under Photobiomodulation Protocols

Abstract

Light-based therapies, such as photobiomodulation, can induce significant thermal effects due to superficial light absorption, particularly in melanin-rich tissues. This study employed artificially pigmented porcine skin to model pigmented skin and evaluate thermal responses under laser exposure. Three therapeutic laser protocols were tested, with continuous temperature monitoring via infrared thermography. Artificial pigmentation significantly increased heat absorption: carbon black caused rises above 20° C, while permanent marker remained under 3° C. Findings emphasize the importance of selecting appropriate pigments in ex vivo thermal safety studies. Future research should incorporate computational modeling and human skin analogs for improved translational relevance.