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Partial purification and some properties of a T2 ribonuclease from Aspergillus flavipes

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dc.contributor.authorGomes, E.
dc.contributor.authorSerzedello, A.
dc.contributor.institutionUniversidade Estadual Paulista (Unesp)
dc.date.accessioned2014-05-20T15:23:54Z
dc.date.available2014-05-20T15:23:54Z
dc.date.issued1996-01-01
dc.description.abstractA ribonuclease was partially purified from the culture medium of Aspergillus flavipes (IZ:1501), after 96 h of cultivation by chromatography on DEAE-cellulose and Sephadex G100 columns. The molecular weight of the RNase was estimated to be 40 kD by gel filtration using Sephadex G100, and the optimum pH and temperature were 4.0 and 50-55 degrees C, respectively. Catalytic activity was inhibited by Zn+2, Fe+3, Hg+2 and Ag+ ions. The enzyme did not show an exact base specificity and produced four kinds of 3'-nucleotides from yeast RNA.en
dc.description.affiliationUNIV ESTADUAL PAULISTA,INST BIOCENCIAS,DEPT BIOQUIM & MICROBIOL,BR-13506900 RIO CLARO,SP,BRAZIL
dc.description.affiliationUnespUNIV ESTADUAL PAULISTA,INST BIOCENCIAS,DEPT BIOQUIM & MICROBIOL,BR-13506900 RIO CLARO,SP,BRAZIL
dc.format.extent227-237
dc.identifierhttp://cat.inist.fr/?aModele=afficheN&cpsidt=2559335
dc.identifier.citationMicrobios. Cambridge: Faculty Press, v. 87, n. 353, p. 227-237, 1996.
dc.identifier.issn0026-2633
dc.identifier.urihttp://hdl.handle.net/11449/34591
dc.identifier.wosWOS:A1996WJ24300003
dc.language.isoeng
dc.publisherFaculty Press
dc.relation.ispartofMicrobios
dc.rights.accessRightsAcesso restrito
dc.sourceWeb of Science
dc.subjectribonucleasept
dc.subjectAspergilluspt
dc.subjectnucleotidespt
dc.titlePartial purification and some properties of a T2 ribonuclease from Aspergillus flavipesen
dc.typeArtigo
dcterms.rightsHolderFaculty Press
dspace.entity.typePublication
unesp.campusUniversidade Estadual Paulista (UNESP), Instituto de Biociências, Rio Claropt
unesp.departmentBioquímica e Microbiologia - IBpt

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Rio Claro - IB - Instituto de Biociências