A radiation hybrid map of bovine X Chromosome (BTAX)
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Springer Nature
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We present a comprehensive radiation hybrid map of the bovine X chromosome (Chr) containing 20 new markers, including both microsatellites and expressed genes. This study was conducted with a 5000-rad whole genome RH cell panel consisting of 90 hybrid cell lines. Retention frequencies of individual markers range from 7.8% for XIST to 31.1% for TGLA325. Statistical analysis with RHMAPPER placed all the loci into five linkage groups under a LOD score criterion of 6.0. These groups could be oriented relative to each other because they included multiple microsatellite loci from the consensus linkage map of the X Chr. Markers included in both this RH map and the bovine cytogenetic map were in a consistent order. The comparative bovine-human map thus generated consists of five blocks of genes, the order of which is conserved, although in the opposite direction when presented as ideograms with p and q arms. Inversions of three blocks account for the difference in gene order across the entirety of the two X Chrs.
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cattle , chromosome inversion , chromosome marker , cytogenetics , gene locus , gene order , genetic conservation , genetic linkage , genome , hybrid cell , nonhuman , radiation hybrid mapping , X chromosome , Animals , Cattle , DNA , Genetic Markers , Humans , Polymerase Chain Reaction , Radiation Hybrid Mapping , X Chromosome , Bos taurus , Bovinae , Mammalia
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Mammalian Genome, v. 13, n. 5, p. 268-271, 2002.
