Chitosan Associated with the Extract of Unripe Banana Peel for Potential Wound Dressing Application

Abstract

Theauthors report the preparation of chitosanmembranes associated with the extract of unripe banana peel. Extractswere prepared by decoction, using boiling distilled water. The extraction process was repeated three times. First and third extracts served as solvent to prepare chitosan membranes by solvent evaporation technique. The chitosan membranes associated with the first and third extracts of unripe banana peel exhibit good flexibility, transparency, and uniformity. Scanning Electron Microscopy images showed dense membranes. Brownish color of membranes was observed due to the presence of tannins, which was confirmed by Infrared Spectroscopy analysis. Thermal properties of the membranes were evaluated by Differential Scanning Calorimetry (DSC), which exhibited good thermal stability at physiological temperature (37 degrees C). At this temperature and pH 7.2, membranes were also able to absorb fluids. Thehydrophilic character of themembranes was determined by contact angle measurements. Themembranes demonstrated their capacity for gaseous exchange and exhibited water-vapor permeability (WVP) rates comparable to injured skin one. Finally, high number of viable dermal fibroblasts was observed by indirect cytotoxicity assay suggesting potential application of these membranes as skin wound dressing.