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In vitro Survival of Follicles Collected from Domestic Cats' Ovaries at Different Stages of Oestrous Cycle and Cultured with IGF-1

dc.contributor.authorAlves, E. A. [UNESP]
dc.contributor.authorPadilha, L.
dc.contributor.authorSavi, P. A.
dc.contributor.authorApparicio, M. F.
dc.contributor.authorMostachio, G. Q.
dc.contributor.authorMotheo, T. F.
dc.contributor.authorPires-Buttler, E. A.
dc.contributor.authorVicente, W. R. R.
dc.contributor.authorLuvoni, G. C.
dc.contributor.institutionUniversidade Estadual Paulista (Unesp)
dc.contributor.institutionUniv Milan
dc.date.accessioned2014-05-20T13:15:21Z
dc.date.available2014-05-20T13:15:21Z
dc.date.issued2012-12-01
dc.description.abstractContents Optimal conditions for in vitro culture of feline ovarian follicles have not yet been defined. Follicular development is regulated by intraovarian growth factors, as insulin-like growth factor (IGF-1), and during the different stages of the oestrous cycle, follicles are exposed to specific hormonal environments. The aim of this study was to investigate the effect of IGF-1 on in vitro growth and granulosa cell (GC) viability of preantral follicles collected from domestic cats at follicular and luteal phases of the oestrous cycle. Oestrus and ovulation were induced in 12 cats. A total of 39 and 32 follicles collected at the follicular and luteal phases, respectively, were individually cultured in vitro for 6 days in minimum essential medium media supplemented with or without IGF-1 (100 ng/ml). Follicles collected during the follicular phase and cultured without IGF-1 displayed a significant increase in size and higher GC viability (46.5 +/- 22.1 mu m, 66.7%, respectively) than that of follicles collected at the luteal phase and cultured without IGF-1 (26.7 +/- 14.4 mu m, 50%, respectively; p < 0.05). In contrast, when IGF-1 was added to the culture medium, no differences were observed in size or GC viability between follicles collected at the two phases of the cycle. Nonetheless, follicles collected at the luteal phase and cultured with IGF-1 had a significant increase in their diameter and GC viability (31.9 +/- 15.9 mu m, 63.6%, respectively) than that cultured without IGF-1 (26.7 +/- 14.4 mu m, 50%, respectively; p < 0.05). These data suggest that in vitro growth and GC survival of feline preantral follicles are affected by the oestrous cycle phase, and the IGF-1 exerts a positive effect on follicles collected at the luteal phase.en
dc.description.affiliationUniv Estadual Paulista, Dept Med Vet Prevent & Reprod Anim, Setor Reprod & Obstet Vet, FCAV,UNESP, BR-14884900 Jaboticabal, SP, Brazil
dc.description.affiliationUniv Milan, Dipartimento Sci Vet Salute Prod Anim & Sicurezza, Milan, Italy
dc.description.affiliationUnespUniv Estadual Paulista, Dept Med Vet Prevent & Reprod Anim, Setor Reprod & Obstet Vet, FCAV,UNESP, BR-14884900 Jaboticabal, SP, Brazil
dc.description.sponsorshipConselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)
dc.format.extent109-112
dc.identifierhttp://dx.doi.org/10.1111/rda.12001
dc.identifier.citationReproduction In Domestic Animals. Hoboken: Wiley-blackwell, v. 47, p. 109-112, 2012.
dc.identifier.doi10.1111/rda.12001
dc.identifier.issn0936-6768
dc.identifier.urihttp://hdl.handle.net/11449/2526
dc.identifier.wosWOS:000312888300024
dc.language.isoeng
dc.publisherWiley-Blackwell
dc.relation.ispartofReproduction in Domestic Animals
dc.relation.ispartofjcr1.422
dc.relation.ispartofsjr0,594
dc.rights.accessRightsAcesso aberto
dc.sourceWeb of Science
dc.titleIn vitro Survival of Follicles Collected from Domestic Cats' Ovaries at Different Stages of Oestrous Cycle and Cultured with IGF-1en
dc.typeArtigo
dcterms.licensehttp://olabout.wiley.com/WileyCDA/Section/id-406071.html
dcterms.rightsHolderWiley-blackwell
dspace.entity.typePublication
unesp.campusUniversidade Estadual Paulista (UNESP), Faculdade de Ciências Agrárias e Veterinárias, Jaboticabalpt
unesp.departmentMedicina Veterinária Preventiva e Reprodução Animal - FCAVpt

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