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Publicação:
Cyclodextrin Production by Bacillus lehensis Isolated from Cassava Starch: Characterisation of a Novel Enzyme

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dc.contributor.authorBlanco, Kate Cristina [UNESP]
dc.contributor.authorMoraes, Flavio Faria de
dc.contributor.authorBernardi, Natalia Sozza [UNESP]
dc.contributor.authorBraga Vettori, Mary Helen Palmuti [UNESP]
dc.contributor.authorMonti, Rubens [UNESP]
dc.contributor.authorContiero, Jonas [UNESP]
dc.contributor.institutionUniversidade Estadual Paulista (Unesp)
dc.contributor.institutionUniversidade Estadual de Maringá (UEM)
dc.date.accessioned2014-12-03T13:11:02Z
dc.date.available2014-12-03T13:11:02Z
dc.date.issued2014-01-01
dc.description.abstractThe properties of a previously unknown enzyme, denominated cyclodextrin glycosyltransferase, produced from Bacillus lehensis, were evaluated using affinity chromatography for protein purification. Enzyme characteristics (optimum pH and temperature; pH and temperature stability), the influence of substances on the enzyme activity, enzyme kinetics, and cyclodextrin production were analysed. Cyclodextrin glycosyltransferase was purified up to 320.74-fold by affinity chromatography using beta-cyclodextrin as the binder and it exhibited 8.71% activity recovery. This enzyme is a monomer with a molecular weight of 81.27 kDa, as estimated by SDS-PAGE. Optimum temperature and pH for cydodextrin glycosyltransferase were 55 degrees C and 8.0, respectively. The Michaelis-Menten constant was 8.62 g/l during maximum velocity of 0.858 g/l.h.en
dc.description.affiliationUNESP Univ Estadual Paulista, Inst Biol Sci, Dept Biochem & Microbiol, BR-1515 Rio Claro, SP, Brazil
dc.description.affiliationUEM Univ Estadual Maringa, Dept Chem Engn, Maringa, Parana, Brazil
dc.description.affiliationUNESP Univ Estadual Paulista, Fac Pharmaceut Sci, Dept Food & Nutr, BR-1515 Rio Claro, SP, Brazil
dc.description.affiliationUnespUNESP Univ Estadual Paulista, Inst Biol Sci, Dept Biochem & Microbiol, BR-1515 Rio Claro, SP, Brazil
dc.description.affiliationUnespUNESP Univ Estadual Paulista, Fac Pharmaceut Sci, Dept Food & Nutr, BR-1515 Rio Claro, SP, Brazil
dc.format.extent48-53
dc.identifierhttp://www.agriculturejournals.cz/web/cjfs.htm?volume=32&firstPage=48&type=publishedArticle
dc.identifier.citationCzech Journal of Food Sciences. Prague: Czech Academy Agricultural Sciences, v. 32, n. 1, p. 48-53, 2014.
dc.identifier.fileWOS000332595800007.pdf
dc.identifier.issn1212-1800
dc.identifier.lattes9859154979447005
dc.identifier.urihttp://hdl.handle.net/11449/112758
dc.identifier.wosWOS:000332595800007
dc.language.isoeng
dc.publisherCzech Academy Agricultural Sciences
dc.relation.ispartofCzech Journal Of Food Sciences
dc.relation.ispartofjcr0.868
dc.relation.ispartofsjr0,355
dc.rights.accessRightsAcesso aberto
dc.sourceWeb of Science
dc.subjectcyclodextrin glycosyltransferaseen
dc.subjectaffinity chromatographyen
dc.subjectpurificationen
dc.titleCyclodextrin Production by Bacillus lehensis Isolated from Cassava Starch: Characterisation of a Novel Enzymeen
dc.typeArtigo
dcterms.rightsHolderCzech Academy Agricultural Sciences
dspace.entity.typePublication
unesp.author.lattes9859154979447005
unesp.campusUniversidade Estadual Paulista (UNESP), Instituto de Biociências, Rio Claropt
unesp.departmentBioquímica e Microbiologia - IBpt

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Rio Claro - IB - Instituto de Biociências