Production of cellulolytic and hemicellulolytic enzymes from Aureobasidium pulluans on solid state fermentation

This article investigates a strain of the yeast Aureobasidium pullulans for cellulase and hemicellulase production in solid state fermentation. Among the substrates analyzed, the wheat bran culture presented the highest enzymatic production (1.05 U/mL endoglucanase, 1.3 U/mL β-glucosidase, and 5.0 U/mL xylanase). Avicelase activity was not detected. The optimum pH and temperature for xylanase, endoglucanase and β-glucosidase were 5.0 and 50, 4.5 and 60, 4.0 and 75°C, respectively. These enzymes remained stable between a wide range of pH. The β-glucosidase was the most thermostable enzyme, remaining 100% active when incubated at 75°C for 1 h. © 2007 Humana Press Inc.

Keywords

β-glucosidase, Cellulases, Endoglucanase, Hemicellulases, Solid state fermentation, Biochemical engineering, Catalysts, Fermentation, Food additives, Proteins, Aureobasidium pullulans, Avicelase activity, Enzymatic production, Glucosidase, Hemicellulase, Hemicellulolytic enzymes, Optimum pH, Wheat bran, Xylanase, Enzymes, beta glucosidase, fungal enzyme, conference paper, enzyme activity, fungal strain, nonhuman, pH, solid state fermentation, thermal stimulation, wheat bran, Ascomycota, Bioreactors, Cell Culture Techniques, Cellulase, Dietary Fiber, Endo-1,4-beta Xylanases, Glycoside Hydrolases, Triticum, Aureobasidium, Triticum aestivum