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Preliminary functional characterization, cloning and primary sequence of Fastuosain, a cysteine peptidase isolated from fruits of Bromelia fastuosa

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dc.contributor.authorCabral, H.
dc.contributor.authorLeopoldino, A. M.
dc.contributor.authorTajara, E. H.
dc.contributor.authorGreene, L. J.
dc.contributor.authorFaca, V. M.
dc.contributor.authorMateus, R. P.
dc.contributor.authorCeron, C. R.
dc.contributor.authorJudice, WAD
dc.contributor.authorJuliano, L.
dc.contributor.authorBonilla-Rodriguez, G. O.
dc.contributor.institutionUniversidade Estadual Paulista (Unesp)
dc.contributor.institutionUniversidade de São Paulo (USP)
dc.contributor.institutionUniversidade Federal de São Paulo (UNIFESP)
dc.date.accessioned2014-05-20T14:03:01Z
dc.date.available2014-05-20T14:03:01Z
dc.date.issued2006-01-01
dc.description.abstractThe present work reports the characterization of Fastuosain, a novel cysteine protease of 25kDa, purified from the unripe fruits of Bromelia fastuosa, a wild South American Bromeliaceae. Proteolytic activity, measured using casein and synthetic substrates, was dependent on the presence of thiol reagents, having maximum activity at pH 7.0. The present work reports cDNA cloning of Fastuosain; cDNA was amplified by PCR using specific primers. The product was 1096pb long. Mature fastuosain has 217 residues, and with the proregion has a total length of 324 residues. Its primary sequence showed high homology with ananain(74%), stem bromelain (66%) and papain (44%).en
dc.description.affiliationUNESP, IBILCE, Dept Quim & Ciências Ambientais, BR-15054000 Sao Jose do Rio Presto, SP, Brazil
dc.description.affiliationUniv São Paulo, Fac Med Ribeirao Preto, Ctr Quim Prot, Ribeirao Preto, SP, Brazil
dc.description.affiliationUNIFESP, Escola Paulista Med, Dept Biofis, São Paulo, Brazil
dc.description.affiliationUnespUNESP, IBILCE, Dept Quim & Ciências Ambientais, BR-15054000 Sao Jose do Rio Presto, SP, Brazil
dc.format.extent83-89
dc.identifierhttp://dx.doi.org/10.2174/092986606774502072
dc.identifier.citationProtein and Peptide Letters. Sharjah: Bentham Science Publ Ltd, v. 13, n. 1, p. 83-89, 2006.
dc.identifier.doi10.2174/092986606774502072
dc.identifier.issn0929-8665
dc.identifier.lattes2307201085881971
dc.identifier.urihttp://hdl.handle.net/11449/22208
dc.identifier.wosWOS:000233942400014
dc.language.isoeng
dc.publisherBentham Science Publ Ltd
dc.relation.ispartofProtein and Peptide Letters
dc.relation.ispartofjcr1.039
dc.relation.ispartofsjr0,429
dc.rights.accessRightsAcesso restrito
dc.sourceWeb of Science
dc.subjectPeptidasept
dc.subjectplant peptidasept
dc.subjectpapainpt
dc.subjectbromelainpt
dc.subjectcysteine-proteasept
dc.subjectproteasept
dc.titlePreliminary functional characterization, cloning and primary sequence of Fastuosain, a cysteine peptidase isolated from fruits of Bromelia fastuosaen
dc.typeArtigo
dcterms.licensehttp://eurekaselect.com/209
dcterms.rightsHolderBentham Science Publ Ltd
dspace.entity.typePublication
unesp.author.lattes2307201085881971
unesp.campusUniversidade Estadual Paulista (UNESP), Instituto de Biociências, Letras e Ciências Exatas, São José do Rio Pretopt
unesp.departmentQuímica e Ciências Ambientais - IBILCEpt

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São José do Rio Preto - IBILCE - Instituto de Biociências, Letras e Ciências Exatas