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Partial purification and some properties of a T2 ribonuclease from Aspergillus flavipes

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dc.contributor.authorGomes, Eleni [UNESP]
dc.contributor.authorSerzëdello, Alcides [UNESP]
dc.contributor.institutionUniversidade Estadual Paulista (UNESP)
dc.date.accessioned2022-04-28T19:54:26Z
dc.date.available2022-04-28T19:54:26Z
dc.date.issued1996-12-01
dc.description.abstractA ribonuclease was partially purified from the culture medium of Aspergillus flavipes (IZ:1501), after 96 h of cultivation by chromatography on DEAE-cellulose and Sephadex G100 columns. The molecular weight of the RNase was estimated to be 40 kD by gel filtration using Sephadex G100, and the optimum pH and temperature were 4.0 and 50-55°C, respectively. Catalytic activity was inhibited by Zn+2, Fe+3, Hg+2 and Ag+ ions. The enzyme did not show an exact base specificity and produced four kinds of 3′-nucleotides from yeast RNA.en
dc.description.affiliationDepartamento de Biologia Inst. Biocencias, Letras Cie. Exatas Universidade Estadual Paulista, Sao Jose do Rio Preto, SP
dc.description.affiliationDepto. de Bioquim. e Microbiologia Instituto de Biocências Universidade Estadual Paulista, CEP 13506-900, Rio Claro, SP
dc.description.affiliationUnespDepartamento de Biologia Inst. Biocencias, Letras Cie. Exatas Universidade Estadual Paulista, Sao Jose do Rio Preto, SP
dc.description.affiliationUnespDepto. de Bioquim. e Microbiologia Instituto de Biocências Universidade Estadual Paulista, CEP 13506-900, Rio Claro, SP
dc.format.extent227-237
dc.identifier.citationMicrobios, v. 87, n. 353, p. 227-237, 1996.
dc.identifier.issn0026-2633
dc.identifier.scopus2-s2.0-0030361622
dc.identifier.urihttp://hdl.handle.net/11449/224044
dc.language.isoeng
dc.relation.ispartofMicrobios
dc.sourceScopus
dc.subjectAspergillus
dc.subjectNucleotides
dc.subjectRibonuclease
dc.titlePartial purification and some properties of a T2 ribonuclease from Aspergillus flavipesen
dc.typeArtigo
dspace.entity.typePublication
unesp.campusUniversidade Estadual Paulista (UNESP), Instituto de Biociências, Rio Claropt
unesp.campusUniversidade Estadual Paulista (UNESP), Instituto de Biociências Letras e Ciências Exatas, São José do Rio Pretopt
unesp.departmentBioquímica e Microbiologia - IBpt
unesp.departmentBiologia - IBILCEpt

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São José do Rio Preto - IBILCE - Instituto de Biociências, Letras e Ciências Exatas
Rio Claro - IB - Instituto de Biociências