Publication: Development of a novel anti-biofilm peptide derived from profilin ofSpodoptera frugiperda
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Coadvisor
Graduate program
Undergraduate course
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Publisher
Taylor & Francis Ltd
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Article
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Abstract
Candidayeast infections are the fourth leading cause of death worldwide. Peptides with antimicrobial activity are a promising alternative treatment for such infections. Here, the antifungal activity of a new antimicrobial peptide-PEP-IA18-was evaluated againstCandidaspecies. PEP-IA18 was designed from the primary sequence of profilin, a protein fromSpodoptera frugiperda,and displayed potent activity againstCandida albicansandCandida tropicalis, showing a minimum inhibitory concentration (MIC) of 2.5 mu M. Furthermore, the mechanism of action of PEP-IA18 involved interaction with the cell membrane (ergosterol complexation). Treatment at MIC and/or 10 x MIC significantly reduced biofilm formation and viability. PEP-IA18 showed low toxicity toward human fibroblasts and only revealed hemolytic activity at high concentrations. Thus, PEP-IA18 exhibited antifungal and anti-biofilm properties with potential applicability in the treatment of infections caused byCandidaspecies.
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Keywords
Antifungal, biofilm, Candida spp, peptide
Language
English
Citation
Biofouling. Abingdon: Taylor & Francis Ltd, 11 p., 2020.
