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Effect of the isocoumarin paepalantine on the luminol and lucigenin amplified chemiluminescence of rat neutrophils

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dc.contributor.authorKitagawa, R. R.
dc.contributor.authorRaddi, MSG
dc.contributor.authorKhalil, N. M.
dc.contributor.authorVilegas, Wagner [UNESP]
dc.contributor.authorda Fonseca, L. M.
dc.contributor.institutionUniversidade Estadual Paulista (Unesp)
dc.date.accessioned2014-05-20T13:24:32Z
dc.date.available2014-05-20T13:24:32Z
dc.date.issued2003-06-01
dc.description.abstractPaepalantine (9,10-dihydroxy-5,7-dimethoxy-1H-naphto(2,3c)pyran-1-one), a natural isocoumarin isolated from the capitula of Paepalanthus bromelioides (Eriocaulaceae), was assessed for its effect on the respiratory burst (zymosan-stimulated luminol-enhanced chemiluminescence and. PMA-stimulated lucigenin-enhanced chemiluminescence) of polymorphonuclear neutrophils in vitro. Special attention was devoted to establishing the IC50 for neutrophils. Paepalantine was able to decrease luminol and lucigenin chemiluminescence, reflecting an inhibitory effect on the respiratory burst, with an ED50 of 0.44 +/- 0.05 and 0.84 +/- 0.15 mug/ml, respectively. A cell-free system was performed with paepalantine on myeloperoxidase/H2O2 and myeloperoxidase/H2O2/Cl- systems. Paepalantine inhibited luminol oxidation in both systems. This inhibition was related to the interaction of paepalantine-myeloperoxidase and its scavenger effect on HOCl.en
dc.description.affiliationUniv Estadual Paulista, Fac Ciências Farmaceut, BR-14801902 Araraquara, SP, Brazil
dc.description.affiliationUniv Estadual Paulista, Inst Quim, BR-14801970 Araraquara, SP, Brazil
dc.description.affiliationUnespUniv Estadual Paulista, Fac Ciências Farmaceut, BR-14801902 Araraquara, SP, Brazil
dc.description.affiliationUnespUniv Estadual Paulista, Inst Quim, BR-14801970 Araraquara, SP, Brazil
dc.format.extent905-908
dc.identifierhttp://dx.doi.org/10.1248/bpb.26.905
dc.identifier.citationBiological & Pharmaceutical Bulletin. Tokyo: Pharmaceutical Soc Japan, v. 26, n. 6, p. 905-908, 2003.
dc.identifier.doi10.1248/bpb.26.905
dc.identifier.fileWOS000183214700032.pdf
dc.identifier.issn0918-6158
dc.identifier.lattes7927877224326837
dc.identifier.orcid0000-0003-3032-2556
dc.identifier.urihttp://hdl.handle.net/11449/7645
dc.identifier.wosWOS:000183214700032
dc.language.isoeng
dc.publisherPharmaceutical Soc Japan
dc.relation.ispartofBiological & Pharmaceutical Bulletin
dc.relation.ispartofjcr1.694
dc.relation.ispartofsjr0,626
dc.rights.accessRightsAcesso aberto
dc.sourceWeb of Science
dc.subjectantioxidant activitypt
dc.subjectpaepalantinept
dc.subjectisocoumarinpt
dc.subjectchemiluminescencept
dc.titleEffect of the isocoumarin paepalantine on the luminol and lucigenin amplified chemiluminescence of rat neutrophilsen
dc.typeArtigo
dcterms.licensehttp://bpb.pharm.or.jp/document/transfer.pdf
dcterms.rightsHolderPharmaceutical Soc Japan
dspace.entity.typePublication
unesp.author.lattes7927877224326837
unesp.author.orcid0000-0003-3032-2556[4]
unesp.campusUniversidade Estadual Paulista (UNESP), Instituto de Biociências, São Vicentept
unesp.departmentCiências Biológicas - IBCLPpt

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São Vicente - IBCLP - Instituto de Biociências