Treinamento físico aeróbio e o eixo de crescimento GH/IGF-1 em ratos diabéticos experimentais
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Data
2005-03-18
Autores
Gomes, Ricardo José [UNESP]
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Editor
Universidade Estadual Paulista (Unesp)
Resumo
Este trabalho teve por objetivo estudar a influencia do treinamento físico aeróbio sobre o eixo de crescimento GH/IGF-1 em ratos diabéticos experimentais. Para o estudo, os ratos foram distribuídos em 4 grupos, controle sedentário (CS), controle treinado (CT), diabético sedentário (DS) e diabético treinado (DT). O diabetes experimental foi induzido por meio de Aloxana (35mg/kg). O programa de treinamento físico consistiu em natação por 60 minutos diários, 5 dias por semana, com uma carga equivalente à 5% do peso corporal, durante 6 semanas consecutivas. Na 5º semana de estudo, os ratos foram submetidos ao teste de tolerância à glicose (GTT), ao teste de tolerância à insulina (ITT) e a uma sessão de exercício agudo. Ao final do período experimental, os ratos foram sacrificados e o sangue foi utilizado para dosagem de glicose, insulina, proteínas, albumina, fosfatase alcalina, hormônio do crescimento (GH), fator de crescimento semelhante à insulina (IGF-1) e proteína transportadora de IGF-1 (IGFBP-3). Amostras do tecido hepático foram utilizadas para avaliação de glicogênio, proteínas totais e DNA, enquanto amostras do músculo gastrocnêmio foram utilizadas para avaliação de glicogênio, proteínas totais, DNA e do peptídeo IGF-1. As tíbias foram retiradas para avaliação do peso, comprimento, densidade mineral e área total. Os resultados obtidos foram avaliados pela análise de variância (ANOVA) com aplicação do teste de Bonferroni, e o nível de significância foi estabelecido em 5%. Para a avaliação das correlações entre variáveis, foi realizada a análise de Spearman's rank order. O diabetes reduziu as concentrações séricas de GH, IGF-1, IGFBP-3, além de reduzir a área total das tíbias no grupo DS...
The purpose of this study was to investigate the influence of aerobic physical training on the GH/IGF-1 growth axis in experimental diabetic rats. For the study, male Wistar rats were distributed into 4 groups, sedentary control (SC), trained control (TC), sedentary diabetic (SD) and trained diabetic (TD). Experimental diabetes was induced by Aloxan (35mg/b.w.) The training program consisted in swimming 5 days/week, 1 h/day, supporting a load of 5% b.w., during 6 weeks. At the 5th week the rats were submitted to glucose (GTT) and insulin (ITT) tolerance tests, and to an acute exercise test. At the end of the experimental period, the rats were sacrificed and the blood was collected for determinations of serum glucose, insulin, proteins, albumin, alkaline phosphatase, growth hormone (GH), insulin-like growth factor (IGF-1) and insulin-like growth factor binding protein-3 (IGFBP-3). Samples of liver tissue were used to evaluate glycogen, protein and DNA contents and samples of muscle (gastrocnemius) were used to evaluate glycogen, protein, DNA and IGF-1 contents. The tibias were collected for weight length, mineral density and total area determination. The results were analyzed by ANOVA, and Bonferroni test and the significance level was set at 5%. To evaluate the relationship between variables, Spearman's rank order analysis was performed. Diabetes decreased serum GH, IGF-1, IGFBP-3 and total tibia area in SD group (P<0.05). Diabetes also resulted in increase in glycaemia and serum alkaline phosphatase. Moreover, there was significant reduction in serum glucose in TD group when compared to SD. Aerobic training promoted increase in serum IGF-1 in both TC and TD groups and increased liver glycogen store... (Complete abstract, click electronic address below)
The purpose of this study was to investigate the influence of aerobic physical training on the GH/IGF-1 growth axis in experimental diabetic rats. For the study, male Wistar rats were distributed into 4 groups, sedentary control (SC), trained control (TC), sedentary diabetic (SD) and trained diabetic (TD). Experimental diabetes was induced by Aloxan (35mg/b.w.) The training program consisted in swimming 5 days/week, 1 h/day, supporting a load of 5% b.w., during 6 weeks. At the 5th week the rats were submitted to glucose (GTT) and insulin (ITT) tolerance tests, and to an acute exercise test. At the end of the experimental period, the rats were sacrificed and the blood was collected for determinations of serum glucose, insulin, proteins, albumin, alkaline phosphatase, growth hormone (GH), insulin-like growth factor (IGF-1) and insulin-like growth factor binding protein-3 (IGFBP-3). Samples of liver tissue were used to evaluate glycogen, protein and DNA contents and samples of muscle (gastrocnemius) were used to evaluate glycogen, protein, DNA and IGF-1 contents. The tibias were collected for weight length, mineral density and total area determination. The results were analyzed by ANOVA, and Bonferroni test and the significance level was set at 5%. To evaluate the relationship between variables, Spearman's rank order analysis was performed. Diabetes decreased serum GH, IGF-1, IGFBP-3 and total tibia area in SD group (P<0.05). Diabetes also resulted in increase in glycaemia and serum alkaline phosphatase. Moreover, there was significant reduction in serum glucose in TD group when compared to SD. Aerobic training promoted increase in serum IGF-1 in both TC and TD groups and increased liver glycogen store... (Complete abstract, click electronic address below)
Descrição
Palavras-chave
Fisiologia, Diabetes Mellitus, Treinamento físico, Physical Training
Como citar
GOMES, Ricardo José. Treinamento físico aeróbio e o eixo de crescimento GH/IGF-1 em ratos diabéticos experimentais. 2005. x, 67 f. Tese (doutorado) - Universidade Estadual Paulista, Instituto de Biociências de Rio Claro, 2005.