Transferência de imunidade passiva de vacas da raça Holandesa imunizadas e não imunizadas contra rotavírus para seus bezerros
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Data
2013-12-17
Autores
Rocha, Thaís Gomes [UNESP]
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Editor
Universidade Estadual Paulista (Unesp)
Resumo
O objetivo do presente estudo foi avaliar a resposta imune humoral de vacas primíparas e pluríparas imunizadas ou não contra rotavírus com vacina comercial inativada e a transferência dessa imunidade para seus bezerros. Para tal, ensaio imunoenzimático indireto (EIE indireto) foi utilizado, sendo constituídos dois grupos e quatro subgrupos experimentais: grupo V1: 24 vacas imunizadas contra rotavírus bovino (BRV), com dois subgrupos - V1n (12 vacas primíparas) e V1v (12 vacas pluríparas) e grupo V2: 24 vacas não imunizadas contra BRV, com dois subgrupos - V2n (12 vacas primíparas) e V2v (12 vacas pluríparas). Os bezerros também foram alocados em grupos e subgrupos, de acordo com a distribuição de suas mães para a avaliação da imunidade humoral sérica, da cinética de parâmetros hematológicos, bioquímicos e da excreção de rotavírus nas fezes nos trinta primeiros dias de vida. Os bezerros foram ainda realocados, de acordo com a ocorrência de diarreia no primeiro mês de vida, sendo constituídos os grupos A (sem diarreia), B (diarreia, negativos para a detecção de rotavírus) e C (diarreia, positivos para rotavírus). Os dados foram submetidos à análise de variância, e o teste de Tukey e o teste de correlação de Pearson foram empregados, considerando-se as diferenças significativas quando P<0,05. Nas vacas dos grupos V1 e V2, não foi verificada influência do número de parições sobre os parâmetros séricos analisados, enquanto que na análise do colostro, verificou-se maiores teores de IgA e IgG em vacas do subgrupo V1v. A imunização pré-parto aumentou significativamente os títulos séricos de IgG anti-BRV, sendo os maiores títulos verificados também nos animais do subgrupo V1v. Os bezerros que receberam colostro dessas vacas (grupo B1) também apresentaram maiores títulos de IgG anti-BRV, quando comparados ao grupo B2, no entanto, a vacinação da vaca...
The aim of this study was to evaluate the humoral immune response of primiparous and multiparous cows immunized with an inactivated commercial vaccine against rotavirus, as well as the passive immunity transfer to its calves through indirect enzymatic immunoassay (EIE); thus, two groups and four subgroups were formed: Group V1: 24 cows immunized with a commercial inactivated vaccine against BRV, comprising two subgroups - V1n (12 first gestation cows) and V1v (12 second or later gestation cows) and Group V2: 24 cows not immunized against BRV, comprising two subgroups - V2n (12 first gestation cows) and V2v (12 second or later gestation cows). The calves were also allotted into groups and subgroups according to its mother’s distribution, in order to evaluate humoral immune response in serum, the kinetics of hematological and biochemical parameters, the acute phase proteins response, and rotavirus excretion in feces throughout the first 30 days of life. The same calves were also redistributed into groups according to the occurrence of diarrhea in the first month of life as Group A (no diarrhea), group B (diarrhea, but negative to rotavirus detection) and group C (diarrhea, positive to rotavirus detection). Data were submitted to variance analysis and Tukey’s test was performed in order to compare mean values of parameters and establish differences between groups; differences were considered significant when P<0,05. Pearson’s correlation test was also performed in some of the studied parameters, and was considered significant when P<0,05. In group V1 and V2 cows, there was no influence of lactation number in the serum parameters analyzed, whilst in the colostrum whey, higher levels of IgA and IgG were noted in V1v cows when compared to the other Subgroups. Prepartum immunization of cows with a commercial vaccine against rotavirus effectively raised titers of specific IgG against rotavirus ...
The aim of this study was to evaluate the humoral immune response of primiparous and multiparous cows immunized with an inactivated commercial vaccine against rotavirus, as well as the passive immunity transfer to its calves through indirect enzymatic immunoassay (EIE); thus, two groups and four subgroups were formed: Group V1: 24 cows immunized with a commercial inactivated vaccine against BRV, comprising two subgroups - V1n (12 first gestation cows) and V1v (12 second or later gestation cows) and Group V2: 24 cows not immunized against BRV, comprising two subgroups - V2n (12 first gestation cows) and V2v (12 second or later gestation cows). The calves were also allotted into groups and subgroups according to its mother’s distribution, in order to evaluate humoral immune response in serum, the kinetics of hematological and biochemical parameters, the acute phase proteins response, and rotavirus excretion in feces throughout the first 30 days of life. The same calves were also redistributed into groups according to the occurrence of diarrhea in the first month of life as Group A (no diarrhea), group B (diarrhea, but negative to rotavirus detection) and group C (diarrhea, positive to rotavirus detection). Data were submitted to variance analysis and Tukey’s test was performed in order to compare mean values of parameters and establish differences between groups; differences were considered significant when P<0,05. Pearson’s correlation test was also performed in some of the studied parameters, and was considered significant when P<0,05. In group V1 and V2 cows, there was no influence of lactation number in the serum parameters analyzed, whilst in the colostrum whey, higher levels of IgA and IgG were noted in V1v cows when compared to the other Subgroups. Prepartum immunization of cows with a commercial vaccine against rotavirus effectively raised titers of specific IgG against rotavirus ...
Descrição
Palavras-chave
Bovino - Vacinação, Bovino de leite, Diarreia em bovino, Resposta imune, Imunoglobulinas, Rotavírus, Immune response
Como citar
ROCHA, Thaís Gomes. Transferência de imunidade passiva de vacas da raça Holandesa imunizadas e não imunizadas contra rotavírus para seus bezerros. 2013. xxiii, 146 p. Tese (doutorado) - Universidade Estadual Paulista, Faculdade de Ciências Agrárias e Veterinárias de Jaboticabal, 2013.