Efeito do sulfato ferroso na desmineralização e remineralização do esmalte bovino: estudos in vitro e in situ
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Data
2009-09-01
Autores
Alves, Karina Mirela Ribeiro Pinto [UNESP]
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Editor
Universidade Estadual Paulista (Unesp)
Resumo
O presente trabalho avaliou o efeito do ferro (Fe) na desmineralização e remineralização de esmalte bovino e pó de hidroxiapatita sintetizada, em estudos in vitro e in situ. Avaliou-se o efeito de diferentes concentrações de Fe (0,33; 0,840; 18,0 e 70,0 μg Fe/mL), na forma de sulfato ferroso, em 3 capítulos distintos: na desmineralização e remineralização do esmalte bovino, usando modelos de ciclagem de pH (capítulo 1); na remineralização de lesões de cárie artificial em esmalte bovino in situ (capítulo 2); e na ciclagem de pH utilizando hidroxiapatita sintetizada (capítulo 3). No primeiro e segundo capítulos, blocos de esmalte tratados com Fe foram avaliados por meio de análise da microdureza de superfície e longitudinal. As análises de F, Ca, P e Fe presentes no esmalte foram determinadas após desmineralização (in vitro) e remineralização (in vitro e in situ). No terceiro capítulo, a análise da hidroxiapatita tratada com soluções de Fe e F foi realizada por meio de espectroscopia no infravermelho transformada de Fourier, além de análises de F, Ca, P e Fe nas soluções de ciclagem de pH. Houve redução da desmineralização do esmalte quando este foi tratado com 18,0 μg Fe/mL(p<0,05); embora, o grupo controle tenha apresentado maior recuperação da dureza na lesão de cárie quando comparado aos grupos tratados com Fe (p<0,05) in vitro. No estudo in situ, nenhuma concentração de Fe promoveu remineralização da lesão de cárie quando comparada ao grupo controle (p<0,05). Um aumento nas concentrações de Fe no esmalte foi observado quando maiores concentrações (18,0 e 70,0 μg Fe/mL) foram utilizadas in vitro e in situ (p<0,05). A taxa Ca/P diminuiu com a presença do Fe na hidroxiapatita (p<0,05) e um aumento do carbonato foi observado na presença...
The present study evaluated the effect of iron (Fe) on enamel demineralization and remineralization of bovine enamel and synthesized hydroxiapatite, using in vitro e in situ models. Different concentrations of Fe, as ferrous sulphate (0.33; 0.840; 18.0 and 70.0 μg Fe/mL) were evaluated in 3 distinct chapters: in bovine enamel demineralization and remineralization, using pH-cycling models (chapter 1); in remineralization of artificial caries lesions on bovine enamel in situ (chapter 2); and in pH cycling using synthesized hydroxiapatite (chapter 3). In the first and second chapters, enamel blocks treated with Fe were evaluated by superficial and longitudinal microhardness. The concentrations of F, Ca, P and Fe present in enamel were also determined after demineralization (in vitro) and remineralization (in vitro and in situ). In the third chapter, the analysis of hydroxyapatite treated with solutions of Fe and F were performed by Fourier Transform Infrared Spectroscopy, ian addition to the analysis of F, Ca, P and Fe in pH cycling solutions. A reduction on enamel demineralization was observed after treatment with 18.0 μg Fe/mL (p<0.05), although the control group presented the greatest recovery of the hardness of caries lesions when compared to groups treated with Fe in vitro (p<0.05). In the in situ study, none of the concentrations evaluated promoted remineralization of caries lesions when compared to the control group (p<0.05). Increases in Fe concentrations in enamel were observed when higher concentrations (18.0 and 70.0 μg Fe/mL) were used in vitro and in situ (p<0.05). The Ca/P rate decreased in the presence of Fe in hydroxyapatite (p<0.05) and an increase of carbonate was observed in the presence of different concentrations of Fe (p<0.05). It was concluded that treatment with a Fecontaining solution... (Complete abstract click electronic access below)
The present study evaluated the effect of iron (Fe) on enamel demineralization and remineralization of bovine enamel and synthesized hydroxiapatite, using in vitro e in situ models. Different concentrations of Fe, as ferrous sulphate (0.33; 0.840; 18.0 and 70.0 μg Fe/mL) were evaluated in 3 distinct chapters: in bovine enamel demineralization and remineralization, using pH-cycling models (chapter 1); in remineralization of artificial caries lesions on bovine enamel in situ (chapter 2); and in pH cycling using synthesized hydroxiapatite (chapter 3). In the first and second chapters, enamel blocks treated with Fe were evaluated by superficial and longitudinal microhardness. The concentrations of F, Ca, P and Fe present in enamel were also determined after demineralization (in vitro) and remineralization (in vitro and in situ). In the third chapter, the analysis of hydroxyapatite treated with solutions of Fe and F were performed by Fourier Transform Infrared Spectroscopy, ian addition to the analysis of F, Ca, P and Fe in pH cycling solutions. A reduction on enamel demineralization was observed after treatment with 18.0 μg Fe/mL (p<0.05), although the control group presented the greatest recovery of the hardness of caries lesions when compared to groups treated with Fe in vitro (p<0.05). In the in situ study, none of the concentrations evaluated promoted remineralization of caries lesions when compared to the control group (p<0.05). Increases in Fe concentrations in enamel were observed when higher concentrations (18.0 and 70.0 μg Fe/mL) were used in vitro and in situ (p<0.05). The Ca/P rate decreased in the presence of Fe in hydroxyapatite (p<0.05) and an increase of carbonate was observed in the presence of different concentrations of Fe (p<0.05). It was concluded that treatment with a Fecontaining solution... (Complete abstract click electronic access below)
Descrição
Palavras-chave
Ferro, Esmalte dentário, Dureza, Remineralização dentária, Desmineralização do dente, Iron, Demineralization, Tooth demineralization, Tooth remineralization, Hardness
Como citar
ALVES, Karina Mirela Ribeiro Pinto. Efeito do sulfato ferroso na desmineralização e remineralização do esmalte bovino: estudos in vitro e in situ. 2009. 116 f. Tese (doutorado) - Universidade Estadual Paulista, Faculdade de Odontologia de Araçatuba, 2009.