Efeito do estresse crônico na modulação da periodontite induzida por ligadura em ratos
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Data
2007-10-01
Autores
Semenoff Segundo, Alex [UNESP]
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Editor
Universidade Estadual Paulista (Unesp)
Resumo
O objetivo do estudo foi comparar o efeito modular do estresse crônico variável e do estresse crônico físico sobre a periodontite induzida por ligadura. Utilizaram-se 48 ratos Wister adultas, divididas aleatoriamente nos grupos: estresse físico GEF (n=2), estresse variável GEV (n=2), controle positivo GCP (n=2) e controle negativo GCN (n=2). Iniciou-se ensaio de estresse físico associado a frio (contenção) e estresse variável (exposição à luz piscante, isolamento, exame da cavidade bucal, ambiente congestionado, odor de sangue e barulho). Decorridos dez dias de estresse, submeteram-se os grupos GEF, GEV e GCP à anestesia, acondicionando fio de seda em volta do segundo molar superior direito, servindo o lado esquerdo com controle. Após processamento laboratorial, realizaram-se a digitalização e a mensuração histométrica das imagens, usando-se dois parâmetros: perda de inserção histológica e perda óssea. No lado com indução de doença, observaram-se maior perda de inserção e perda óssea para GEF, comparado aos demais grupos. O GEV não apresentou diferenças estatísticas, comparado ao grupo GCP. O lado, sem indução de doença, demonstrou ausência de diferença estatística. Em relação ao peso do timo, o GEV teve um aumento e diferenciou-se do GCP e GCN. O timo do GEF também aumentou, diferenciando-se do GCP. O peso das adrenais diminuiu no GEF, diferenciando-se do GCP e GCN. Com relação ao peso do fígado as diferenças estatísticas foram notadas no GEF, se comparado ao GCP e ao GCN. Também se observou diferença estatística entre GEV e GCN. Concluindo, o estresse físico modulou o padrão de resposta da periodontite induzida.
Stress is an intrinsic factor in modern society, though its relation with periodontal disease is contraversial. In the light of this problem, the modulatory effect of variable chronic stress and chronic physical stress on ligature-induced periodontitis was compared. Forty eight adult Wister rats were used, randomly divided into four groups: physical stress (PSG n=2), variable stress (VSG n=2), control positive (PC n=2), and control negative (NC n=2). Experiments for physical stress, immobilization associated with cold, and variable stress, including flickering light exposure, isolation, buccal cavity exam, overcrowded environment, blood odor and noise, were realized. After ten days of stress, the PSG, VSG and PC groups were anesthetized and a suture thread was placed around the upper right second molar, with the left side serving as control. The experimental results revealed a greater loss of insertion and bone destruction for the PSG compared to the remaining groups. The VSG in relation to the PC, though with no statistical difference. On the left side with no ligature-induced disease, no statistical differences were observed between the groups. The thymus was found to be enlarged in the VSG, differentiating this group from the PC and NC. The thymus in the PSG was different from the PC. The adrenals were diminished in the PSG, differentiating this group from the PC and NC. Statistical differences were found in the liver between the PSG and the PC and NC, with differences also observed between the VSG and NC. In conclusion, stress models physical modulated the standard response to ligature-induced periodontitis in rats.
Stress is an intrinsic factor in modern society, though its relation with periodontal disease is contraversial. In the light of this problem, the modulatory effect of variable chronic stress and chronic physical stress on ligature-induced periodontitis was compared. Forty eight adult Wister rats were used, randomly divided into four groups: physical stress (PSG n=2), variable stress (VSG n=2), control positive (PC n=2), and control negative (NC n=2). Experiments for physical stress, immobilization associated with cold, and variable stress, including flickering light exposure, isolation, buccal cavity exam, overcrowded environment, blood odor and noise, were realized. After ten days of stress, the PSG, VSG and PC groups were anesthetized and a suture thread was placed around the upper right second molar, with the left side serving as control. The experimental results revealed a greater loss of insertion and bone destruction for the PSG compared to the remaining groups. The VSG in relation to the PC, though with no statistical difference. On the left side with no ligature-induced disease, no statistical differences were observed between the groups. The thymus was found to be enlarged in the VSG, differentiating this group from the PC and NC. The thymus in the PSG was different from the PC. The adrenals were diminished in the PSG, differentiating this group from the PC and NC. Statistical differences were found in the liver between the PSG and the PC and NC, with differences also observed between the VSG and NC. In conclusion, stress models physical modulated the standard response to ligature-induced periodontitis in rats.
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Palavras-chave
Periodontite, Periodontitis -Rats
Como citar
SEMENOFF SEGUNDO, Alex. Efeito do estresse crônico na modulação da periodontite induzida por ligadura em ratos. 2007. 116 f. Tese (doutorado) - Universidade Estadual Paulista, Faculdade de Odontologia de Araçatuba, 2007.