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dc.contributor.authorMachado, Gabriel Capella
dc.contributor.authorMoris, Daniela Vanessa
dc.contributor.authorArantes, Thales Domingos
dc.contributor.authorSilva, Luciane Regina Franciscone
dc.contributor.authorTheodoro, Raquel Cordeiro
dc.contributor.authorMendes, Rinaldo Poncio [UNESP]
dc.contributor.authorVicentini, Adriana Pardini
dc.contributor.authorBagagli, Eduardo
dc.date.accessioned2014-10-01T13:08:41Z
dc.date.available2014-10-01T13:08:41Z
dc.date.issued2013-08-01
dc.identifierhttp://dx.doi.org/10.1590/0074-0276108052013016
dc.identifier.citationMemórias do Instituto Oswaldo Cruz. Instituto Oswaldo Cruz, Ministério da Saúde, v. 108, n. 5, p. 637-643, 2013.
dc.identifier.issn0074-0276
dc.identifier.urihttp://hdl.handle.net/11449/109942
dc.description.abstractWe aimed to evaluate whether the occurrence of cryptic species of Paracoccidioides brasiliensis, S1, PS2, PS3 and Paracoccidioides lutzii, has implications in the immunodiagnosis of paracoccidioidomycosis (PCM). Small quantities of the antigen gp43 were found in culture filtrates of P. lutzii strains and this molecule appeared to be more variable within P. lutzii because the synonymous-nonsynonymous mutation rate was lower, indicating an evolutionary process different from that of the remaining genotypes. The production of gp43 also varied between isolates belonging to the same species, indicating that speciation events are important, but not sufficient to fully explain the diversity in the production of this antigen. The culture filtrate antigen AgEpm83, which was obtained from a PS3 isolate, showed large quantities of gp43 and reactivity by immunodiffusion assays, similar to the standard antigen (AgB-339) from an S1 isolate. Furthermore, AgEpm83 was capable of serologically differentiating five serum samples from patients from the Botucatu and Jundiaí regions. These patients had confirmed PCM but, were non-reactive to the standard antigen, thus demonstrating an alternative for serological diagnosis in regions in which S1 and PS2 occur. We also emphasise that it is not advisable to use a single antigen preparation to diagnose PCM, a disease that is caused by highly diverse pathogens.en
dc.format.extent637-643
dc.language.isoeng
dc.publisherInstituto Oswaldo Cruz, Ministério da Saúde
dc.relation.ispartofMemórias do Instituto Oswaldo Cruz
dc.sourceSciELO
dc.subjectParacoccidioides sppen
dc.subjectcryptic speciesen
dc.subjectantigen preparationsen
dc.subjectimmunodiagnosisen
dc.subjectgp43en
dc.titleCryptic species of Paracoccidioides brasiliensis: impact on paracoccidioidomycosis immunodiagnosisen
dc.typeArtigo
dc.contributor.institutionUniversidade Estadual Paulista (UNESP)
dc.contributor.institutionInstituto Adolfo Lutz (IAL)
dc.description.affiliationUniversidade Estadual Paulista Julio de Mesquita Filho Instituto de Biociencias de Botucatu Departamento de Microbiologia e Imunologia
dc.description.affiliationUniversidade Estadual Paulista Julio de Mesquita Filho Faculdade de Medicina de Botucatu Departamento de Doencas Tropicais
dc.description.affiliationInstituto Adolfo Lutz Centro de Imunologia Laboratorio de Imunodiagnostico das Micoses
dc.description.affiliationUnespUniversidade Estadual Paulista Julio de Mesquita Filho Instituto de Biociencias de Botucatu Departamento de Microbiologia e Imunologia
dc.description.affiliationUnespUniversidade Estadual Paulista Julio de Mesquita Filho Faculdade de Medicina de Botucatu Departamento de Doencas Tropicais
dc.identifier.doi10.1590/0074-0276108052013016
dc.identifier.scieloS0074-02762013000500637
dc.identifier.scieloS0074-0276108052013016
dc.identifier.wosWOS:000322520800016
dc.rights.accessRightsAcesso aberto
dc.identifier.scopus2-s2.0-84879583133
unesp.campusUniversidade Estadual Paulista (UNESP), Instituto de Biociências, Botucatupt
unesp.campusUniversidade Estadual Paulista (UNESP), Faculdade de Medicina, Botucatupt
dc.identifier.fileS0074-02762013000500637.pdf
dc.identifier.lattes3320327570429539
unesp.author.lattes3320327570429539
unesp.author.orcid0000-0001-5270-6302[3]
unesp.author.orcid0000-0002-9321-0909[7]
unesp.author.orcid0000-0001-5016-1046[5]
dc.relation.ispartofjcr2.833
dc.relation.ispartofsjr1,172
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