A liquid-phase-blocking concanavalin A enzyme-linked immunosorbent assay for the detection of antibodies against Newcastle disease virus in serum of free-ranging pigeons
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Data
2013-11-01
Autores
Oliveira, Elisabete Schirato de [UNESP]
Silva, Ketherson Rodrigues [UNESP]
Fernando, Filipe Santos [UNESP]
Mello Goncalves, Mariana Costa [UNESP]
Fernandes, Camila Cesario [UNESP]
Borzi, Mariana Monezi [UNESP]
Santos, Romeu Moreira dos [UNESP]
Feres Tamanini, Maria de Lourdes [UNESP]
Silva Montassier, Maria de Fatima da [UNESP]
Montassier, Helio Jose [UNESP]
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Editor
Sage Publications Inc
Resumo
A competitive liquid-phase-blocking concanavalin A enzyme-linked immunosorbent assay (LPB-ConA-ELISA) was developed in the current study. The assay used ConA as a capture reagent, and the sera of specific pathogen-free chickens immunized with nonpurified Newcastle disease virus (NDV) suspension as detector antibodies, to detect and quantify specific antiviral antibodies in serum samples from free-ranging pigeons. The comparison between the LPB-ConA-ELISA and the hemagglutination inhibition (HI) test for the detection of antibodies in serum samples from 107 pigeons showed significant correlation between the assays (r = 0.875), a high sensitivity (100%), specificity (95.8%), accuracy (96.3%) for the ELISA, and good agreement ( = 0.83) between the 2 assays. The results of this study suggest that the LPB-ConA-ELISA could be a useful alternative to HI test in the serodiagnosis of NDV in pigeons, or other species of birds.
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Palavras-chave
Immunodiagnostic, lectins, Newcastle disease virus, pigeons
Como citar
Journal Of Veterinary Diagnostic Investigation. Thousand Oaks: Sage Publications Inc, v. 25, n. 6, p. 720-726, 2013.