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dc.contributor.authorAlves, Cleidson Padua
dc.contributor.authorFonseca, Aline Simoneti
dc.contributor.authorMuys, Bruna Rodrigues
dc.contributor.authorBueno, Rafaela de Barros e Lima
dc.contributor.authorBuerger, Matheus Carvalho
dc.contributor.authorSouza, Jorge E. S. de
dc.contributor.authorValente, Valeria [UNESP]
dc.contributor.authorZago, Marco Antonio
dc.contributor.authorSilva, Wilson Araujo
dc.identifier.citationStem Cells. Hoboken: Wiley-blackwell, v. 31, n. 12, p. 2827-2832, 2013.
dc.description.abstractHotair is a member of the recently described class of noncoding RNAs called lincRNA (large intergenic noncoding RNA). Various studies suggest that Hotair acts regulating epigenetic states by recruiting chromatin-modifying complexes to specific target sequences that ultimately leads to suppression of several genes. Although Hotair has been associated with metastasis and poor prognosis in different tumor types, a deep characterization of its functions in cancer is still needed. Here, we investigated the role of Hotair in the scenario of epithelial-to-mesenchymal transition (EMT) and in the arising and maintenance of cancer stem cells (CSCs). We found that treatment with TGF-1 resulted in increased Hotair expression and triggered the EMT program. Interestingly, ablation of Hotair expression by siRNA prevented the EMT program stimulated by TGF-1, and also the colony-forming capacity of colon and breast cancer cells. Furthermore, we observed that the colon CSC subpopulation (CD133(+)/CD44(+)) presents much higher levels of Hotair when compared with the non-stem cell subpopulation. These results indicate that Hotair acts as a key regulator that controls the multiple signaling mechanisms involved in EMT. Altogether, our data suggest that the role of Hotair in tumorigenesis occurs through EMT triggering and stemness acquisition.en
dc.description.sponsorshipFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
dc.description.sponsorshipConselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)
dc.description.sponsorshipCoordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)
dc.relation.ispartofStem Cells
dc.sourceWeb of Science
dc.subjectCancer stem cellen
dc.subjectEpithelial-to-mesenchymal transitionen
dc.titleBrief Report: The lincRNA Hotair Is Required for Epithelial-to-Mesenchymal Transition and Stemness Maintenance of Cancer Cell Linesen
dc.contributor.institutionUniversidade de São Paulo (USP)
dc.contributor.institutionNatl Inst Sci & Technol Stem Cell & Cell Therapy
dc.contributor.institutionCtr Cell Based Therapy
dc.contributor.institutionUniversidade Estadual Paulista (UNESP)
dc.contributor.institutionInst Bioinformat & Biotechnol 2Bio
dc.description.affiliationUniv Sao Paulo, Dept Genet, Ribeirao Preto Med Sch, Sao Paulo, Brazil
dc.description.affiliationUniv Sao Paulo, Dept Internal Med, Ribeirao Preto Med Sch, Sao Paulo, Brazil
dc.description.affiliationNatl Inst Sci & Technol Stem Cell & Cell Therapy, Sao Paulo, Brazil
dc.description.affiliationCtr Cell Based Therapy, Sao Paulo, Brazil
dc.description.affiliationUniv Estadual Paulista, Fac Pharmaceut Sci Araraquara, Sao Paulo, Brazil
dc.description.affiliationUniv Sao Paulo, Ctr Integrat Syst Biolo CISBi, NAP, BR-09500900 Sao Paulo, Brazil
dc.description.affiliationInst Bioinformat & Biotechnol 2Bio, Sao Paulo, Brazil
dc.description.affiliationUnespUniv Estadual Paulista, Fac Pharmaceut Sci Araraquara, Sao Paulo, Brazil
dc.rights.accessRightsAcesso restrito
dc.description.sponsorshipIdFAPESP: 12/00588-5
dc.description.sponsorshipIdFAPESP: 98/14247-6
unesp.campusUniversidade Estadual Paulista (UNESP), Faculdade de Ciências Farmacêuticas, Araraquarapt
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