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dc.contributor.authorGasparotto, Vinicius P. O. [UNESP]
dc.contributor.authorLandim-Alvarenga, Fernanda C. [UNESP]
dc.contributor.authorOliveira, Alexandre L. R. [UNESP]
dc.contributor.authorSimoes, Gustavo Ferreira
dc.contributor.authorLima-Neto, Joao F. [UNESP]
dc.contributor.authorBarraviera, Benedito [UNESP]
dc.contributor.authorFerreira, Rui S. [UNESP]
dc.date.accessioned2014-12-03T13:10:32Z
dc.date.available2014-12-03T13:10:32Z
dc.date.issued2014-06-10
dc.identifierhttp://dx.doi.org/10.1186/scrt467
dc.identifier.citationStem Cell Research & Therapy. London: Biomed Central Ltd, v. 5, 10 p., 2014.
dc.identifier.issn1757-6512
dc.identifier.urihttp://hdl.handle.net/11449/112229
dc.description.abstractIntroduction: The optimization of an organic scaffold for specific types of applications and cells is vital to successful tissue engineering. In this study, we investigated the effects of a new fibrin sealant derived from snake venom as a scaffold for mesenchymal stem cells, to demonstrate the ability of cells to affect and detect the biological microenvironment.Methods: The characterization of CD34, CD44 and CD90 expression on mesenchymal stem cells was performed by flow cytometry. In vitro growth and cell viability were evaluated by light and electron microscopy. Differentiation into osteogenic, adipogenic and chondrogenic lineages was induced.Results: The fibrin sealant did not affect cell adhesion, proliferation or differentiation and allowed the adherence and growth of mesenchymal stem cells on its surface. Hoechst 33342 and propidium iodide staining demonstrated the viability of mesenchymal stem cells in contact with the fibrin sealant and the ability of the biomaterial to maintain cell survival.Conclusions: The new fibrin sealant is a three-dimensional scaffolding candidate that is capable of maintaining cell survival without interfering with differentiation, and might also be useful in drug delivery. Fibrin sealant has a low production cost, does not transmit infectious diseases from human blood and has properties of a suitable scaffold for stem cells because it permits the preparation of differentiated scaffolds that are suitable for every need.en
dc.description.sponsorshipFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
dc.description.sponsorshipConselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)
dc.description.sponsorshipCoordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)
dc.format.extent10
dc.language.isoeng
dc.publisherBiomed Central Ltd.
dc.relation.ispartofStem Cell Research & Therapy
dc.sourceWeb of Science
dc.titleA new fibrin sealant as a three-dimensional scaffold candidate for mesenchymal stem cellsen
dc.typeArtigo
dcterms.rightsHolderBiomed Central Ltd
dc.contributor.institutionUniversidade Estadual Paulista (UNESP)
dc.contributor.institutionUniversidade Estadual de Campinas (UNICAMP)
dc.description.affiliationUniv Estadual Paulista, UNESP, Sao Paulo State Univ, Dept Trop Dis & Image Diag,Botucatu Med Sch, BR-18618970 Sao Paulo, Brazil
dc.description.affiliationUniv Estadual Paulista, UNESP, Sao Paulo State Univ, Ctr Study Venoms & Venomous Anim UNESP,CEVAP, BR-18610307 Sao Paulo, Brazil
dc.description.affiliationUniv Estadual Paulista, UNESP, Sao Paulo State Univ, Dept Anim Reprod & Vet Radiol,Sch Vet Med & Anim, BR-18618970 Sao Paulo, Brazil
dc.description.affiliationUniv Estadual Campinas, UNICAMP, Dept Struct & Funct Biol, Lab Nerve Regenerat, BR-13083865 Sao Paulo, Brazil
dc.description.affiliationUnespUniv Estadual Paulista, UNESP, Sao Paulo State Univ, Dept Trop Dis & Image Diag,Botucatu Med Sch, BR-18618970 Sao Paulo, Brazil
dc.description.affiliationUnespUniv Estadual Paulista, UNESP, Sao Paulo State Univ, Ctr Study Venoms & Venomous Anim UNESP,CEVAP, BR-18610307 Sao Paulo, Brazil
dc.description.affiliationUnespUniv Estadual Paulista, UNESP, Sao Paulo State Univ, Dept Anim Reprod & Vet Radiol,Sch Vet Med & Anim, BR-18618970 Sao Paulo, Brazil
dc.identifier.doi10.1186/scrt467
dc.identifier.wosWOS:000338465200001
dc.rights.accessRightsAcesso aberto
dc.description.sponsorshipIdFAPESP: 09/53846-9
dc.description.sponsorshipIdFAPESP: 09/06280-0
dc.description.sponsorshipIdFAPESP: 12/08101-8
dc.description.sponsorshipIdCNPq: 563582/2010-3
dc.description.sponsorshipIdCAPES: 1219/2011
dc.description.sponsorshipIdCAPES: 23038.000823/2011-21
unesp.campusUniversidade Estadual Paulista (UNESP), Faculdade de Medicina, Botucatupt
unesp.campusUniversidade Estadual Paulista (UNESP), Faculdade de Medicina Veterinária e Zootecnia, Botucatupt
dc.identifier.fileWOS000338465200001.pdf
unesp.author.orcid0000-0002-9855-5594[6]
dc.relation.ispartofjcr4.963
dc.relation.ispartofsjr1,685
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