In vitro evaluation of adherence of Candida albicans, Candida glabrata, and Streptococcus mutans to an acrylic resin modified by experimental coatings
Nenhuma Miniatura disponível
Data
2014-05-28
Autores
Izumida, Fernanda Emiko [UNESP]
Moffa, Eduardo Buozi [UNESP]
Vergani, Carlos Eduardo [UNESP]
Machado, Ana Lucia [UNESP]
Jorge, Janaina Habib [UNESP]
Giampaolo, Eunice Teresinha [UNESP]
Título da Revista
ISSN da Revista
Título de Volume
Editor
Taylor & Francis Ltd
Resumo
This study evaluated the effect of experimental coatings, containing zwitterion or hydrophilic monomers, on the adherence of Candida albicans, Candida glabrata, and Streptococcus mutans to an acrylic resin. Acrylic samples (smooth or rough surfaces) were left untreated (control) or coated with one of the following experimental coatings: 3-hydroxypropylmethacrylate (HP) or sulfobetaine methacrylate (S), at concentrations of 25, 30, or 35%. Half of the specimens were coated with saliva. The adhesion test was performed by incubating specimens in C. albicans, C. glabrata, and S. mutans suspensions at 37 degrees C for 90 min. The number of adhered microorganisms was determined by metabolic activity (XTT) and by cell viability (CFU). All coated specimens exhibited lower absorbance and CFU values compared to control specimens. Saliva and roughness did not promote microorganism adherence. An XPS analysis confirmed the modification in the chemical composition of the coatings in the experimental samples. These experimental coatings significantly reduced the adherence of C. albicans, C. glabrata and S. mutans to acrylic resin.
Descrição
Palavras-chave
Candida albicans, saliva, acrylic resin, Candida glabrata, Streptococcus mutans
Como citar
Biofouling. Abingdon: Taylor & Francis Ltd, v. 30, n. 5, p. 525-533, 2014.