Identificação de regiões genômicas selecionadas de forma divergente em equinos quarto de milha de corrida e trabalho
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2014-06-26
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Universidade Estadual Paulista (Unesp)
Resumo
Caracterizada por sua grande versatilidade em várias modalidades equestres, a raça Quarto de Milha se destaca mundialmente, representando 52% de toda população equina no mundo. Dentro da raça há subdivisão em diferentes segmentos de aptidão, provenientes de distintos objetivos de seleção, consideradas linhagens, entre elas corrida e trabalho. Objetivou-se com este estudo avaliar as diferenças morfológicas e genômicas que ocorrem entre as linhagens de corrida e trabalho como resultado da seleção para diferentes objetivos, a identificação de regiões genômicas que tenham sido alteradas pela seleção na formação da linhagem de corrida em relação à linhagem de trabalho e realizar dois estudos, independentes, de associação ampla do genoma (GWAS) para identificar regiões cromossômicas e genes candidatos posicionais associados com características de desempenho na linhagem de corrida e com características morfométricas na raça como um todo. Para as análises foram utilizados 120 animais de corrida e 64 animais de trabalho de ambos os sexos registrados na Associação Brasileira de Criadores de Cavalo Quarto de Milha. As características morfométricas avaliadas foram: peso, altura à cernelha, comprimentos corporal, da canela, da quartela, da garupa, da cabeça, e do pescoço, perímetros torácico, da canela e do casco e a característica de desempenho índice de velocidade. A análise das diferenças morfológicas foi realizada por meio do procedimento GLM do programa SAS utilizando-se modelo que incluiu os efeitos de sexo e linhagem e a covariável idade à mensuração. Para a análise das diferenças genômicas, 54.602 SNPs foram genotipados utilizando-se o painel de marcadores Illumina Equine SNP50 BeadChip. Os resultados obtidos revelaram mudanças significativas entre as linhagens para todas as características morfométricas avaliadas. Animais de corrida apresentaram maiores pesos, alturas, comprimentos ...
Characterized by its versatility in several sporting activities, the Quarter Horse breed excels globally, representing 52% of the entire equine population in the world. The Quarter Horse breed is subdivided into different lines according to skills resulting from distinct selection objectives, including cutting and racing horses. The aims of the present study were to investigate the morphological and genomic difference, between cutting and racing lines as a result of selection for different objectives; identification of the genomic regions divergently selected in racing line in relation to cutting line and to perform two genome-wide association studies, independently, to identify chromosomal regions and positional candidate genes associated with performance trait in the racing line and morphometric traits in the breed as a whole. To perform the analyses 120 racing animals and 64 cutting animals of both sexes and registered at the Brazilian Association of Quarter Horse Breeders were used. The evaluated morphometric traits were: weight; height at withers; length of the body, shank, pastern, rump, head, and neck; circumference of the chest, shank, and hoof and speed index performance trait. Morphological differences analysis was performed using a model that included the fixed effects of sex and line, and age at recording as covariate. The GLM procedure of the SAS program was used for statistical analysis. To perform genomic differences analysis, 54,602 SNPs were genotyped by the Illumina Equine SNP50 BeadChip array. The results showed significant changes in the morphometric traits of the animals. Racing animals were heavier and taller and presented greater body lengths and perimeters than cutting horses. The number of informative SNPs and the SNP density found in the genome of cutting and racing animals suggest that the Equine SNP50 BeadChip can be used for different purposes in the Quarter Horse breed. To identify genomic regions ...
Characterized by its versatility in several sporting activities, the Quarter Horse breed excels globally, representing 52% of the entire equine population in the world. The Quarter Horse breed is subdivided into different lines according to skills resulting from distinct selection objectives, including cutting and racing horses. The aims of the present study were to investigate the morphological and genomic difference, between cutting and racing lines as a result of selection for different objectives; identification of the genomic regions divergently selected in racing line in relation to cutting line and to perform two genome-wide association studies, independently, to identify chromosomal regions and positional candidate genes associated with performance trait in the racing line and morphometric traits in the breed as a whole. To perform the analyses 120 racing animals and 64 cutting animals of both sexes and registered at the Brazilian Association of Quarter Horse Breeders were used. The evaluated morphometric traits were: weight; height at withers; length of the body, shank, pastern, rump, head, and neck; circumference of the chest, shank, and hoof and speed index performance trait. Morphological differences analysis was performed using a model that included the fixed effects of sex and line, and age at recording as covariate. The GLM procedure of the SAS program was used for statistical analysis. To perform genomic differences analysis, 54,602 SNPs were genotyped by the Illumina Equine SNP50 BeadChip array. The results showed significant changes in the morphometric traits of the animals. Racing animals were heavier and taller and presented greater body lengths and perimeters than cutting horses. The number of informative SNPs and the SNP density found in the genome of cutting and racing animals suggest that the Equine SNP50 BeadChip can be used for different purposes in the Quarter Horse breed. To identify genomic regions ...
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Equino, Polimorfismo (Genetica), Nucleotídeos, Genetica animal, Genomas, Desempenho, Genomes
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MEIRA, Camila Tângari. Identificação de regiões genômicas selecionadas de forma divergente em equinos quarto de milha de corrida e trabalho. 2014. vi, 95 p. Tese (doutorado) - Universidade Estadual Paulista Júlio de Mesquita Filho, Faculdade de Ciências Agrárias e Veterinárias, 2014.