Effect of cell cycle synchronization on the accuracy of murine and bovine embryo sex determination
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Data
1994-02-01
Autores
Lima, V. F. M. Hossepian de
Bem, A. R. de
Jorge, W. [UNESP]
Moreira-Filho, C. A.
Título da Revista
ISSN da Revista
Título de Volume
Editor
Butterworth-heinemann
Resumo
Different cell cycle synchronization methods were used to increase the mitotic index and accuracy of sex determination in murine and bovine embryos. For sexing purposes, colchicine treatment for 2, 4, 6 and 8 h and the FdU-thymidine-colchicine combination were tested in murine embryos. The best results were obtained with colchicine treatment for 8 h (96.88% accuracy) and with FdU-thymidine-colchicine (97.22% accuracy). Mitotic indexes differed significantly between the 2 treatments (21.71% for colchicine and 32.95% for FdU-thymidine-colchicine). For sex identification of murine and bovine demi-embryos, both treatments were demonstrated to be equally effective (nearly 90%). The mitotic index for the FdU-treated murine demi-embryos (19.04%) was higher than the one obtained for the 8-h colchicine treatment (15.62%).
Descrição
Palavras-chave
Colchicine, Embryo, Fluordeoxyuridine, Sexing, Bovinae, Murinae
Como citar
Theriogenology. Woburn: Butterworth-heinemann, v. 41, n. 2, p. 521-534, 1994.