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dc.contributor.authorQuishida, Cristiane Campos Costa [UNESP]
dc.contributor.authorOliveira Mima, Ewerton Garcia de[UNESP]
dc.contributor.authorDovigo, Lívia Nordi [UNESP]
dc.contributor.authorJorge, Janaina Habib [UNESP]
dc.contributor.authorBagnato, Vanderlei Salvador
dc.contributor.authorPavarina, Ana Cláudia [UNESP]
dc.date.accessioned2015-12-07T15:38:23Z
dc.date.available2015-12-07T15:38:23Z
dc.date.issued2015-09-24
dc.identifierhttp://dx.doi.org/10.1007/s10103-015-1811-9
dc.identifier.citationLasers In Medical Science, p. 1-10, 2015.
dc.identifier.issn1435-604X
dc.identifier.urihttp://hdl.handle.net/11449/131601
dc.description.abstractIn this investigation, the effectiveness of successive applications of antimicrobial photodynamic inactivation (API) mediated by Photodithazine(®) (PDZ) and LED light was evaluated against a multispecies biofilm formed by Candida albicans, Candida glabrata, and Streptococcus mutans on denture base acrylic resin. Standard cell suspensions (bacteria and yeast) were inoculated on acrylic resin samples, and the biofilm was grown for 48 h (37 °C/75 rpm). API was performed by the administration of PDZ (175 and 200 mg/L) and exposure to 37.5 J/cm(2) of LED light (660 nm). Additional samples were treated with PDZ or LED light only. Untreated control samples were not submitted to light or PDZ. The conditions described were applied once or in three consecutive applications for all groups. Cell viability was determined by colony counts (CFU/mL), metabolic activity, total biomass, and confocal laser scanning microscopy (CLSM). Data were analyzed by a nonparametric two-way ANOVA and Tukey tests (α = 0.05). The results obtained demonstrated a significant effect (p < 0.05) of number of applications and treatment groups for CFU/mL, and S. mutans showed the highest susceptibility to API. The metabolic activity of the multispecies biofilm was significantly reduced (p < 0.05) after API for both numbers of applications, which were also significantly different (p < 0.05) between them. The total biomass of the biofilm was significantly different (p < 0.05) only between groups submitted to one and three API applications. CLSM showed a visual increase of dead cells after API. API-mediated PDZ was effective in reducing the cell viability of multispecies biofilm. Three consecutive applications of API were more effective for reducing the cell viability and the total biomass of multispecies biofilm.en
dc.description.sponsorshipFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
dc.description.sponsorshipFundação para o Desenvolvimento da UNESP (FUNDUNESP)
dc.description.sponsorshipCentro de Pesquisa em Óptica e Fotônica (CEPOF)
dc.format.extent1-10
dc.language.isoeng
dc.publisherSpringer
dc.relation.ispartofLasers In Medical Science
dc.sourcePubMed
dc.subjectAcrylic resinen
dc.subjectBiofilmen
dc.subjectCandida albicansen
dc.subjectCandida glabrataen
dc.subjectPhotodynamic therapyen
dc.subjectStreptococcus mutansen
dc.titlePhotodynamic inactivation of a multispecies biofilm using Photodithazine(®) and LED light after one and three successive applicationsen
dc.typeArtigo
dcterms.rightsHolderSpringer
dc.contributor.institutionUniversidade Estadual Paulista (UNESP)
dc.contributor.institutionUniversidade de São Paulo (USP)
dc.description.affiliationDepartment of Dental Materials and Prosthodontics, Araraquara Dental School, UNESP, Univ Estadual Paulista, Rua Humaitá 1680, CEP 14801-903, Araraquara, SP, Brazil. criscquishida@gmail.com.
dc.description.affiliationDepartment of Dental Materials and Prosthodontics, Araraquara Dental School, UNESP, Univ Estadual Paulista, Rua Humaitá 1680, CEP 14801-903, Araraquara, SP, Brazil.
dc.description.affiliationDepartment of Social Dentistry, Araraquara Dental School, UNESP - Univ Estadual Paulista, Rua Humaitá 1680, CEP 14801-903, Araraquara, SP, Brazil.
dc.description.affiliationDepartment of Dental Materials and Prosthodontics, Araraquara Dental School, UNESP, Univ Estadual Paulista, Rua Humaitá 1680, CEP 14801-903, Araraquara, SP, Brazil.
dc.description.affiliationPhysics Institute of São Carlos, USP, University of São Paulo, Av. Trabalhador São-Carlense, 400, CEP: 13566-590, São Carlos, SP, Brazil.
dc.description.affiliationDepartment of Dental Materials and Prosthodontics, Araraquara Dental School, UNESP, Univ Estadual Paulista, Rua Humaitá 1680, CEP 14801-903, Araraquara, SP, Brazil.
dc.description.affiliationUnespDepartment of Dental Materials and Prosthodontics, Araraquara Dental School, UNESP, Univ Estadual Paulista, Rua Humaitá 1680, CEP 14801-903, Araraquara, SP, Brazil.
dc.description.affiliationUnespDepartment of Dental Materials and Prosthodontics, Araraquara Dental School, UNESP, Univ Estadual Paulista, Rua Humaitá 1680, CEP 14801-903, Araraquara, SP, Brazil.
dc.description.affiliationUnespDepartment of Social Dentistry, Araraquara Dental School, UNESP - Univ Estadual Paulista, Rua Humaitá 1680, CEP 14801-903, Araraquara, SP, Brazil.
dc.description.affiliationUnespDepartment of Dental Materials and Prosthodontics, Araraquara Dental School, UNESP, Univ Estadual Paulista, Rua Humaitá 1680, CEP 14801-903, Araraquara, SP, Brazil.
dc.description.affiliationUnespDepartment of Dental Materials and Prosthodontics, Araraquara Dental School, UNESP, Univ Estadual Paulista, Rua Humaitá 1680, CEP 14801-903, Araraquara, SP, Brazil.
dc.identifier.doi10.1007/s10103-015-1811-9
dc.rights.accessRightsAcesso restrito
dc.description.sponsorshipIdFAPESP: 2011/09054
dc.description.sponsorshipIdCEPOF: 13/07276-1
dc.description.sponsorshipIdFUNDUNESP: 878/11-DFP
unesp.campusUniversidade Estadual Paulista (UNESP), Faculdade de Odontologia, Araraquarapt
dc.identifier.lattes8867670539105403
dc.identifier.lattes0127570480681342
dc.identifier.pubmed26404782
unesp.author.lattes0127570480681342
unesp.author.lattes8867670539105403[5]
unesp.author.orcid0000-0002-9135-3455[3]
unesp.author.orcid0000-0002-9231-1994[5]
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