Influência do tabagismo nos níveis de beta-defensina 3 no fluido crevicular gengival de indivíduos com periodontite crônica
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Data
2018-03-16
Autores
Marcantonio, Ana Carolina Monachini
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Editor
Universidade Estadual Paulista (Unesp)
Resumo
O propósito deste estudo foi determinar a influência do tabagismo nos níveis de beta-defensina 3 (hBD 3) no fluido crevicular gengival (FCG) de indivíduos com periodontite crônica e avaliar sua relação com saúde e doença periodontal. Além disso, foi investigada a correlação deste peptídeo antimicrobiano com metaloproteinase da matriz (MMP-8) e citocinas pró (IL-10) e anti-inflamatórias (IL-1β, IFN-γ e TNF-α). Um total de 40 indivíduos com periodontite crônica, sendo 20 fumantes (PF) e 20 não fumantes (PNF), e 20 indivíduos sem doença periodontal (S) foram incluídos no estudo. Amostras de FCG de sítios sadios e doentes dos indivíduos com periodontite, e apenas de sítios sadios dos indivíduos periodontalmente saudáveis, foram coletadas com tiras de papel absorvente. A quantificação da hBD 3 foi feita pela técnica ELISA sanduíche e dos marcadores biológicos por ensaio Multiplex. Níveis significativamente menores de hBD 3 foram identificados nos sítios doentes de PF em comparação aos sítios doentes de PNF (p=0,02). Por outro lado, os níveis de hBD 3 nos sítios sadios de PF foram significativamente maiores que nos sítios sadios de PNF e S (p=0,006). Na comparação dos sítios dentro do grupo PF, foi verificado que os sítios doentes apresentavam níveis reduzidos de hBD 3 em comparação com sítios sadios (p=0,04). Já no grupo PNF, os níveis de hBD 3 foram mais elevados nos sítios doentes que nos sítios sadios (p=0,02). Uma correlação negativa foi observada entre os níveis de hBD 3 e MMP-8, IL-1β e IFN-γ tanto nos sítios sadios como nos sítios doentes de PF e PNF, e uma correlação positiva foi verificada entre hBD 3 e IL-10. Baseado nestes resultados, concluímos que o fumo teve um impacto negativo na expressão da hBD3 no fluido crevicular gengival de pacientes com periodontite crônica. Esta redução nos níveis da hBD 3 poderia ser mais um mecanismo envolvido na associação entre tabagismo e doença periodontal.
The purpose of this study was to determine the influence of smoking on gingival crevicular fluid (GCF) levels of beta-defensin 3 (hBD 3) in individuals with chronic periodontitis and to evaluate its relationship with health and periodontal disease. In addition, the correlation of this antimicrobial peptide with matrix metalloproteinase (MMP-8) and pro (IL-10) and anti-inflammatory cytokines (IL-1β, IFN-γ and TNF-α) was investigated. A total of 40 individuals with chronic periodontitis, including 20 smokers (PS) and 20 non-smokers (PNS), and 20 subjects without periodontal disease (H) were included in the study. GCF samples from healthy and diseased sites of individuals with periodontitis, and only from healthy sites of periodontally healthy individuals, were collected with absorbent paper strips. Quantification of hBD 3 was performed by a sandwich ELISA assay and the biological markers levels were analyzed by a multiplex assay. Significantly lower levels of hBD 3 were identified in diseased sites of PS compared to diseased sites of PNS (p = 0.02). On the other hand, the levels of hBD 3 in healthy sites of PS were significantly higher than in healthy sites of PNS and H (p = 0.006). The comparison between the sites within the groups with periodontitis showed reduced levels of hBD 3 in diseased sites of PS compared to healthy sites (p = 0.04), while higher levels of this peptide was detected in diseased sites of PNS compared to healthy sites (p = 0.02). A negative correlation was observed between the levels of hBD 3 and MMP-8, IL-1β and IFN-γ in healthy and diseased sites of PS and PNS, and a positive correlation was found between hBD 3 and IL-10. Based on these results, we concluded that smoking had a negative impact on hBD 3 expression in gingival crevicular fluid of patients with chronic periodontitis. This reduction on hBD 3 levels may be an additional mechanism on smoking and periodontal disease association.
The purpose of this study was to determine the influence of smoking on gingival crevicular fluid (GCF) levels of beta-defensin 3 (hBD 3) in individuals with chronic periodontitis and to evaluate its relationship with health and periodontal disease. In addition, the correlation of this antimicrobial peptide with matrix metalloproteinase (MMP-8) and pro (IL-10) and anti-inflammatory cytokines (IL-1β, IFN-γ and TNF-α) was investigated. A total of 40 individuals with chronic periodontitis, including 20 smokers (PS) and 20 non-smokers (PNS), and 20 subjects without periodontal disease (H) were included in the study. GCF samples from healthy and diseased sites of individuals with periodontitis, and only from healthy sites of periodontally healthy individuals, were collected with absorbent paper strips. Quantification of hBD 3 was performed by a sandwich ELISA assay and the biological markers levels were analyzed by a multiplex assay. Significantly lower levels of hBD 3 were identified in diseased sites of PS compared to diseased sites of PNS (p = 0.02). On the other hand, the levels of hBD 3 in healthy sites of PS were significantly higher than in healthy sites of PNS and H (p = 0.006). The comparison between the sites within the groups with periodontitis showed reduced levels of hBD 3 in diseased sites of PS compared to healthy sites (p = 0.04), while higher levels of this peptide was detected in diseased sites of PNS compared to healthy sites (p = 0.02). A negative correlation was observed between the levels of hBD 3 and MMP-8, IL-1β and IFN-γ in healthy and diseased sites of PS and PNS, and a positive correlation was found between hBD 3 and IL-10. Based on these results, we concluded that smoking had a negative impact on hBD 3 expression in gingival crevicular fluid of patients with chronic periodontitis. This reduction on hBD 3 levels may be an additional mechanism on smoking and periodontal disease association.
Descrição
Palavras-chave
Periodontite crônica, beta-Defensinas, Tabagismo, Citocinas, Chronic periodontitis, beta-Defensins, Tobacco use disorder, Cytokines