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dc.contributor.authorFinato, Angela Carolina [UNESP]
dc.contributor.authorFraga-Silva, Thais Fernanda [UNESP]
dc.contributor.authorUliana Carvalho Prati, Amanda [UNESP]
dc.contributor.authorAlves de Souza, Amauri [UNESP]
dc.contributor.authorMazzeu, Bruna Fonseca [UNESP]
dc.contributor.authorFelippe, Lidiane Gaspareto [UNESP]
dc.contributor.authorPinto, Rute Alves [UNESP]
dc.contributor.authorDe Assis Golim, Marjorie [UNESP]
dc.contributor.authorSueli Parreira Arruda, Maria [UNESP]
dc.contributor.authorFurlan, Maysa [UNESP]
dc.contributor.authorVenturini, James [UNESP]
dc.date.accessioned2018-12-11T17:37:30Z
dc.date.available2018-12-11T17:37:30Z
dc.date.issued2018-06-01
dc.identifierhttp://dx.doi.org/10.1371/journal.pone.0198682
dc.identifier.citationPLoS ONE, v. 13, n. 6, 2018.
dc.identifier.issn1932-6203
dc.identifier.urihttp://hdl.handle.net/11449/179970
dc.description.abstractIn this study, we aimed to evaluate the immunomodulatory effects of crude leaf extracts from Piper gaudichaudianum Kunth, P. arboreum Aub., P. umbellata L., P. fuligineum Kunth, and Peperomia obtusifolia A. Dietr. on an in vitro model of inflammatory response. The crude extracts were previously obtained by maceration of the leaves. The half-maximal inhibitory concentration was determined by the MTT assay using human peripheral blood mononuclear cells. Human monocytes were simultaneously challenged with each crude extract and lipopolysaccharide (LPS), the major component of the outer membrane of Gram-negative bacteria, to induce a strong inflammatory response. After 24 h of incubation, cell-free supernatants were used for evaluating the mediators involved in inflammation: H2O2, TNF-α, IL-8, IL-6, IL-1β, IL-10, IL-12, FGF-b, and TGF-β1. We also compared the results with the effects of ketoprofen, a well-known anti-inflammatory drug. The P. gaudichaudianum crude extract downmodulated the production of H2O2, IL-1β, IL-6, IL-8, and TGF-β1 by LPS-stimulated monocytes; P. arboreum, IL-1β, IL-6, IL-8, and TNF-α; P. umbellata and P. fuligineum, H2O2, IL-1β, IL-6, IL-8, IL-10, and TNF-α; and P. obtusifolia, H2O2, IL-6, IL-8, IL-10, and TNF-α. In general, the crude leaf extracts amplified the anti-inflammatory response when compared with ketoprofen, particularly reducing the production of IL-8, a mediator involved in neutrophil recruitment during tissue damage. Thus, the crude leaf extracts of P. gaudichaudianum, P. arboreum, P. umbellata, P. fuligineum, and Peperomia obtusifolia elicited an anti-inflammatory response against LPS-challenged monocytes. These findings show the anti-inflammatory properties of these crude leaf extracts and offer new perspectives for their use in the treatment of inflammatory diseases.en
dc.description.sponsorshipFundação de Apoio à Pesquisa do Estado da Paraíba
dc.language.isoeng
dc.relation.ispartofPLoS ONE
dc.sourceScopus
dc.titleCrude leaf extracts of piperaceae species downmodulate inflammatory responses by human monocytesen
dc.typeArtigo
dc.contributor.institutionUniversidade Estadual Paulista (UNESP)
dc.contributor.institutionUniversidade Federal de Mato Grosso do Sul (UFMS)
dc.description.affiliationUniversidade Estadual Paulista (Unesp) Faculdade de Ciências
dc.description.affiliationUniversidade Estadual Paulista (Unesp) Instituto de Biociências
dc.description.affiliationUniversidade Estadual Paulista (Unesp) Instituto de Química
dc.description.affiliationUniversidade Estadual Paulista (Unesp) Faculdade de Medicina
dc.description.affiliationUniversidade Federal de Mato Grosso do Sul (UFMS) Faculdade de Medicina
dc.description.affiliationUnespUniversidade Estadual Paulista (Unesp) Faculdade de Ciências
dc.description.affiliationUnespUniversidade Estadual Paulista (Unesp) Instituto de Biociências
dc.description.affiliationUnespUniversidade Estadual Paulista (Unesp) Instituto de Química
dc.description.affiliationUnespUniversidade Estadual Paulista (Unesp) Faculdade de Medicina
dc.identifier.doi10.1371/journal.pone.0198682
dc.rights.accessRightsAcesso aberto
dc.description.sponsorshipIdFundação de Apoio à Pesquisa do Estado da Paraíba: 2016/17627-4
dc.identifier.scopus2-s2.0-85048821515
dc.identifier.file2-s2.0-85048821515.pdf
dc.relation.ispartofsjr1,164
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