Efeito de vernizes fluoretados suplementados com trimetafosfato de sódio nanoparticulado sobre o desgaste erosivo do esmalte dental in situ.
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Data
2020-10-28
Autores
Silva, Isabela Ferreira da [UNESP]
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Universidade Estadual Paulista (Unesp)
Resumo
O presente estudo avaliou o efeito de vernizes fluoretados suplementados com micro- ou nanopartículas de Trimetafosfato de Sódio (TMP) sobre o desgaste erosivo do esmalte dental bovino, utilizando um protocolo in situ/ex vivo, duplo-cego e cruzado. Blocos de esmalte dental bovino (n=224) foram selecionados por meio de dureza de superfície (DS) e divididos aleatoriamente em 4 grupos experimentais de acordo com os vernizes testados: (a) Placebo (sem NaF ou TMP), (b) 5% NaF, (c) 5% NaF + 5% TMP microparticulado (5% Micro) e (d) 5% NaF + 5% TMP nanoparticulado (5% Nano). Os blocos foram inseridos em dispositivos acrílicos palatinos (n=4/dispositivo) e tratados com os vernizes uma única vez. Após 6 h na cavidade bucal dos voluntários (n=14), os vernizes foram removidos e os blocos, submetidos a desafios erosivos (ERO, imersão ex vivo em ácido cítrico 0,05 M, pH 3,2, 90 s, 4x/dia). Em seguida, dois blocos de cada dispositivo foram adicionalmente submetidos a abrasão por escovação com dentifrício fluoretado (ERO+ABR), totalizando 5 dias em cada etapa experimental. Os blocos foram analisados por dureza de superfície (DS) e perfilometria. Os dados foram submetidos a ANOVA a dois critérios e Teste de Fisher LSD (p<0,05). O menor desgaste foi observado para o grupo 5% Nano, seguido por 5% Micro < 5% NaF < Pla, com diferenças significativas entre todos os grupos. A maior perda de DS foi observada para o grupo Pla, e a menor para o 5% Nano (ERO/ERO+ABR), sem diferenças significativas entre Pla e 5% NaF (ERO), 5% NaF e 5% Micro (ERO+ABR), e 5% Micro e 5% Nano (ERO+ABR). Diante dos resultados, conclui-se que a adição de 5% de TMP a um verniz fluoretado convencional aumentou significativamente a proteção contra o desgaste erosivo do esmalte, e a utilização de nanopartículas deste fosfato aumentou ainda mais este efeito.
The present study evaluated the effect of fluoride varnishes containing micrometric or nano-sized Sodium Trimetaphosphate (TMP) on enamel erosive wear, following an in situ/ex vivo, double-blind and crossover protocol. Bovine dental enamel blocks (n= 224) were selected by surface hardness (SH) and divided into 4 experimental groups according to the varnishes tested: (a) Placebo (without NaF or TMP), (b) 5% NaF, (c) 5% NaF + 5% micrometric TMP (5% Micro) and (d) 5% NaF + 5% nano-sized TMP (5% Nano). The blocks were inserted in acrylic palatal devices (n=4/device), received a single varnish application and remained in the oral cavity of the subjects (n=14) for 6 h. Afterwards, the varnishes were removed and the blocks submitted to erosive challenges (ERO, immersion ex vivo in 0.05 M citric acid, pH 3.2, 90 s, 4x/ day). Then, two blocks of each device were additionally submitted to abrasion by brushing with fluoride dentifrice (ERO + ABR), totaling 5 days in each experimental phase. All blocks were analyzed by profilometry and SH, and the data, submitted to 2-way ANOVA and Fisher’s LSD test (p < 0.05). The lowest wear was observed for 5% Nano, followed by 5% Micro < 5% NaF < Pla, with significant differences among all groups. The highest SH loss was observed for Pla and the lowest for 5% Nano (ERO/ERO+ABR), with no significant differences between Pla and 5% NaF (ERO), 5% NaF and 5% Micro (ERO+ABR), 5% Micro and 5% Nano (ERO+ABR). In view of the results, it was concluded that the addition of 5% TMP to a conventional fluoride varnish increased the protection against erosive enamel wear, and the use of nanoparticles further enhanced this effect.
The present study evaluated the effect of fluoride varnishes containing micrometric or nano-sized Sodium Trimetaphosphate (TMP) on enamel erosive wear, following an in situ/ex vivo, double-blind and crossover protocol. Bovine dental enamel blocks (n= 224) were selected by surface hardness (SH) and divided into 4 experimental groups according to the varnishes tested: (a) Placebo (without NaF or TMP), (b) 5% NaF, (c) 5% NaF + 5% micrometric TMP (5% Micro) and (d) 5% NaF + 5% nano-sized TMP (5% Nano). The blocks were inserted in acrylic palatal devices (n=4/device), received a single varnish application and remained in the oral cavity of the subjects (n=14) for 6 h. Afterwards, the varnishes were removed and the blocks submitted to erosive challenges (ERO, immersion ex vivo in 0.05 M citric acid, pH 3.2, 90 s, 4x/ day). Then, two blocks of each device were additionally submitted to abrasion by brushing with fluoride dentifrice (ERO + ABR), totaling 5 days in each experimental phase. All blocks were analyzed by profilometry and SH, and the data, submitted to 2-way ANOVA and Fisher’s LSD test (p < 0.05). The lowest wear was observed for 5% Nano, followed by 5% Micro < 5% NaF < Pla, with significant differences among all groups. The highest SH loss was observed for Pla and the lowest for 5% Nano (ERO/ERO+ABR), with no significant differences between Pla and 5% NaF (ERO), 5% NaF and 5% Micro (ERO+ABR), 5% Micro and 5% Nano (ERO+ABR). In view of the results, it was concluded that the addition of 5% TMP to a conventional fluoride varnish increased the protection against erosive enamel wear, and the use of nanoparticles further enhanced this effect.
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Palavras-chave
Fluoretos, Polifosfatos, Erosão dentária, Fluorides