Diferential gene expression in sugar cane infected with Xanthomonas albilineans, causal agent of leaf scald
Nenhuma Miniatura disponível
Data
2006-01-01
Autores
Dabbas, Karina Maia [UNESP]
Ferro, Maria Inês Tiraboschi [UNESP]
de Barros, Neli Martins [UNESP]
de Laia, Marcelo Luiz [UNESP]
Zingaretti, Sonia Marli [UNESP]
Giachetto, Poliana Fernanda [UNESP]
de Moraes, Vicente Alberto
Ferro, Jesus Aparecido [UNESP]
Título da Revista
ISSN da Revista
Título de Volume
Editor
Resumo
The leaf scald disease, caused by the xylem-invading pathogen Xanthomonas albilineans, is one of the most devastating diseases of sugarcane. Chronic symptoms are characterized by white pencil-line streaks surrounding invaded leaf vascular bundles, which may result in fully burnt leaves, and by lateral bud development. In this work, using a macroarray approach, we analyzed the expression profile of 3,575 ESTs (expressed sequence tags) of sugarcane, in two sugarcane varieties, classified as susceptible (SP78-4467) or resistant (SP82-1176) to Xanthomonas albilineans infection. Membranes were constructed with ESTs from sugarcane leaf roll tissues and hybridized with radio labeled cDNAs from infected and non-infected sugarcane tissue. Data analysis showed a different pattern of expression during pathogen infection. This approach allowed the identification of differentially induced ESTs for proteins involved isoprenoid biosynthesis, transmembrane LRR protein, leucine zipper, lignification, cold tolerance, plant response and environmental adaptation in the resistant variety. Repressed ESTs in this variety were related to proteins involved in plant cellular expansion, detoxification, and auxin transport. In the susceptible variety, the differentially repressed ESTs were related to proteins involved in plant defense response, Ethylene biosynthesis, and transcription regulation.
Descrição
Palavras-chave
Avirulence genes, cDNA, Host-pathogen interaction, Macroarray, Resistence genes
Como citar
Summa Phytopathologica, v. 32, n. 4, p. 328-338, 2006.