Ação antibiofilme de Zingiber officinale contra Acinetobacter baumannii e Klebsiella pneumoniae
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Data
2022-07-08
Autores
Sousa, Isabella Gomes
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Universidade Estadual Paulista (Unesp)
Resumo
Esta pesquisa avaliou a ação antimicrobiana do extrato glicólico de Z. officinale contra A. baumannii e K. pneumoniae na forma planctônica e biofilmes monotípicos. Material e Métodos: foi determinada a Concentração Inibitória Mínima (CIM) e a Concentração Microbicida Mínima (CMM) pelo teste de microdiluição em caldo, segundo a Clinical and Laboratory Standards Institute (CLSI). Posteriormente, foi realizada a verificação da atividade antimicrobiana sobre biofilmes monotípicos e a redução da atividade metabólica foi avaliada pelo teste de MTT. Para isso, as concentrações de uma, duas e quatro vezes a CIM, foram utilizadas para o tratamento dos biofilmes no tempo de 5 min e 24h. Os dados obtiveram distribuição normal sendo a análise estatística realizada por ANOVA e Tukey (p<0,05%). Resultados: O extrato promoveu CIM e CMM de 0,39 mg/mL para todas as cepas multirresistentes de K. pneumoniae testadas e 0,78 mg/mL para todas as cepas de A. baumannii. A redução do biofilme variou conforme a concentração e cepa quando tratado com o extrato, sendo a cepa K. pneumoniae ATCC 4352 com maior índice de redução de 53,1%. Conclusão: Pode-se concluir que o extrato glicólico de Z. officinale apresentou ação bactericida na forma planctônica das bactérias testadas, e apresentou ação antibiofilme apenas para a cepa K. pneumoniae ATCC 4352 no tratamento de 24h na concentração 1,56mg/mL e na cepa clínica 58004 de A. baumannii, no tratamento de 5 min na concentração 0,78mg/mL.
This research evaluated the antimicrobial action of the glycolic extract of Z. officinale against A. baumannii and K. pneumoniae in planktonic form and monotypic biofilms. Material and Methods: Minimum Inhibitory Concentration (MIC) and Minimum Microbicidal Concentration (MMC) were determined by the broth microdilution test, according to the Clinical and Laboratory Standards Institute (CLSI). Subsequently, the verification of antimicrobial activity on monotypic biofilms was performed and the reduction of metabolic activity was evaluated by the MTT test. For this, the concentration of one, two and four times the MIC were used for the treatment of biofilms in the time of 5 min and 24 h. The data obtained a normal distribution and the statistical analysis was performed by ANOVA and Tukey (p<0.05%). Results: The extract promoted MIC and MMC of 0.39 mg/mL for all multidrug-resistant strains of K. pneumoniae tested and 0.78 mg/mL for all A. baumannii strains. Biofilm reduction varied according to concentration and strain when treated with the extract, with the K. pneumoniae ATCC 4352 strain having the highest reduction rate of 53.1%. Conclusion: It can be concluded that the glycolic extract of Z. officinale showed bactericidal action in the planktonic form of the bacteria tested, and showed antibiofilm action only for the strain K. pneumoniae ATCC 4352 in the 24h treatment at the concentration of 1.56mg/mL and in the clinical strain 58004 of A. baumannii, in the 5 min treatment at a concentration of 0.78mg/mL.
This research evaluated the antimicrobial action of the glycolic extract of Z. officinale against A. baumannii and K. pneumoniae in planktonic form and monotypic biofilms. Material and Methods: Minimum Inhibitory Concentration (MIC) and Minimum Microbicidal Concentration (MMC) were determined by the broth microdilution test, according to the Clinical and Laboratory Standards Institute (CLSI). Subsequently, the verification of antimicrobial activity on monotypic biofilms was performed and the reduction of metabolic activity was evaluated by the MTT test. For this, the concentration of one, two and four times the MIC were used for the treatment of biofilms in the time of 5 min and 24 h. The data obtained a normal distribution and the statistical analysis was performed by ANOVA and Tukey (p<0.05%). Results: The extract promoted MIC and MMC of 0.39 mg/mL for all multidrug-resistant strains of K. pneumoniae tested and 0.78 mg/mL for all A. baumannii strains. Biofilm reduction varied according to concentration and strain when treated with the extract, with the K. pneumoniae ATCC 4352 strain having the highest reduction rate of 53.1%. Conclusion: It can be concluded that the glycolic extract of Z. officinale showed bactericidal action in the planktonic form of the bacteria tested, and showed antibiofilm action only for the strain K. pneumoniae ATCC 4352 in the 24h treatment at the concentration of 1.56mg/mL and in the clinical strain 58004 of A. baumannii, in the 5 min treatment at a concentration of 0.78mg/mL.
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Acinetobacter baumannii, antibacterianos, Klebsiella pneumoniae, medicamentos fitoterápicos, Zingiber officinale, antibacterials, herbal medicines