Influência do uso de diferentes crioprotetores e número de palhetas sobre o tempo de congelamento
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Data
2022
Autores
Capuzzo, Caio de Souza
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Resumo
A taxa de congelamento é um dos processos que causam grandes mudanças na
célula, e a busca por taxas de congelamento ótimas pode minimizar fatores
prejudiciais desse processo. Neste trabalho o objetivo foi determinar as curvas de
congelamento de dois equipamentos para criopreservação: caixa de poliestireno
(EPS) e Dry Shipper. Testou-se duas soluções crioprotetoras com diferentes números
de palhetas e duas soluções de diluentes (a base de gema e BTS). Foram feitas cinco
repetições com duas, quatro, oito e 12 palhetas na caixa de isopor e Dry Shipper, foi
cronometrado o tempo até atingir -80 °C. O equipamento Dry Shipper influenciou
significativamente sobre o tempo de congelamento ao se utilizar diferentes
combinações de números de paletas, e os crioprotetores a base de gema e BTS
influenciaram sobre este tempo na parte de cima penas. Na caixa de isopor, os dois
crioprotetores afetaram significativamente sobre o tempo de congelamento na
distância de 1 cm do nitrogênio enquanto que na distância de 3 cm foram as diferentes
quantidades de palhetas. A solução com gema foi a que influenciou nos menores
tempos de congelamento.
The freezing rate is one of the processes that cause big changes in the cell, and the search for optimal freezing rates can minimize harmful factors of this process. In this work, the objective was to determine the freezing curves of two cryopreservation equipment: polystyrene box (EPS) and Dry Shipper. Two cryoprotectant solutions with different numbers of straws and two diluent solutions (yolk based and BTS) were tested. Five repetitions were made with two, four, eight and 12 straws in the styrofoam box and Dry Shipper, the time until reaching -80 °C was timed. The Dry Shipper equipment significantly influenced the freezing time when using different combinations of pallet numbers, and the yolk-based cryoprotectants and BTS influenced this time on the top feathers. In the styrofoam box, the two cryoprotectants significantly affected the freezing time at a distance of 1 cm from nitrogen, while at a distance of 3 cm were the different amounts of straws. The yolk solution was the one that influenced the lowest freezing times.
The freezing rate is one of the processes that cause big changes in the cell, and the search for optimal freezing rates can minimize harmful factors of this process. In this work, the objective was to determine the freezing curves of two cryopreservation equipment: polystyrene box (EPS) and Dry Shipper. Two cryoprotectant solutions with different numbers of straws and two diluent solutions (yolk based and BTS) were tested. Five repetitions were made with two, four, eight and 12 straws in the styrofoam box and Dry Shipper, the time until reaching -80 °C was timed. The Dry Shipper equipment significantly influenced the freezing time when using different combinations of pallet numbers, and the yolk-based cryoprotectants and BTS influenced this time on the top feathers. In the styrofoam box, the two cryoprotectants significantly affected the freezing time at a distance of 1 cm from nitrogen, while at a distance of 3 cm were the different amounts of straws. The yolk solution was the one that influenced the lowest freezing times.
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Palavras-chave
Aquicultura, Congelamento de sêmen, Criogenia, Dry Shipper, Piscicultura, Reprodução, Aquaculture, Semen freezing, Cryogenics, Pisciculture, Reproduction