Extração de esterase de fígado suíno (PLE)
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Data
2006-07-01
Autores
Trevisan, Henrique Celso [UNESP]
Medeiros, João Batista de [UNESP]
Lisboa, Helen Cristina Fávero [UNESP]
Título da Revista
ISSN da Revista
Título de Volume
Editor
Sociedade Brasileira de Química
Resumo
A simple, fast and low-cost methodology was optimized, seeking preparation of a crude pig liver esterase (PLE) concentrate. Basically, the method consisted of the following steps: liver homogenization, acetone washing, enzyme extraction and purification/concentration. Starting from 1 kg of fresh liver more than 200 kU of PLE suspension were obtained after 8 hours, at an estimated cost of US$0.21/kU. The PLE concentrate thus obtained was stable, showing 96-100% of the initial activity after 7 months in refrigerator at 4°C.
Descrição
Palavras-chave
porcine liver esterase, PLE, biocatalysis
Como citar
Química Nova. Sociedade Brasileira de Química, v. 29, n. 4, p. 865-867, 2006.