Similaridade genética entre acessos de goiabeiras e araçazeiros baseada em marcadores moleculares AFLP
Carregando...
Data
2011-09-01
Título da Revista
ISSN da Revista
Título de Volume
Editor
Sociedade Brasileira de Fruticultura
Resumo
Espécies do gênero Psidium, como goiabeira e araçazeiros, são economicamente importantes e têm como área de diversidade genética primária o Brasil. Foram determinadas as relações genéticas, com base no marcador AFLP, para acessos do Banco Ativo de Germoplasma (BAG) de Psidium da Empresa Brasileira de Pesquisa Agropecuária (EMBRAPA) Semiárido, para orientar trabalhos de melhoramento e de manejo de recursos genéticos do gênero. Foram analisados 88 acessos, sendo 64 de goiabeira e 24 de araçazeiros, coletados em dez Estados brasileiros, adotando-se para agrupamento o dendrograma UPGMA, considerando a matriz de similaridade do coeficiente de Jaccard de 149 bandas polimórficas de AFLP de 16 combinações dos iniciadores EcoRI e MseI. A análise da variância de dados moleculares foi realizada considerando a variação entre e dentro das populações de goiabeira dos dez Estados. O dendrograma apresentou boa definição, com coeficiente de correlação cofenética de 0,94. Foram observados dois grandes grupos: um formado por acessos de goiabeira e outro com acessos de araçazeiros, com inclusão de alguns acessos de goiabeira. Foram observados agrupamentos específicos no dendograma apenas para os indivíduos de goiabeira coletados em Goiás e Roraima. Os acessos estudados apresentaram similaridade variando de 28 a 98%, evidenciando a alta variabilidade genética dos mesmos. A variação entre acessos foi estimada em 0,16 (ΦST), indicando diferenciação genética moderada entre as populações de goiabeira dos dez Estados. Para aumentar a variabilidade genética do BAG estudado, sugere-se a coleta de um número maior de acessos nos Estados de Goiás e Roraima, dado o alto índice de similaridade entre os acessos provenientes destes Estados, bem como coletas amplas em outros Estados brasileiros. Sugerem-se ainda cruzamentos entre os poucos acessos de goiabeira posicionados no grupo dos araçazeiros para o desenvolvimento de híbridos interespecificos no gênero Psidium.
Species of the genus Psidium, like guava and Brazilian guava trees, are economically important and have the area of primary genetic diversity in Brazil. It was determined the genetic relationships based on AFLP marker for accessions of Psidium Germplasm Bank (PGB) of Empresa Brasileira de Pesquisa Agropecuária (EMBRAPA) Semiarid to guide improvements and management of genetic resources of the genus. Eighty-eight accessions were analyzed, 64 of guava and 24 of Brazilian guava, collected in ten Brazilian States, adopting for the cluster dendrogram UPGMA, considering the similarity matrix of Jaccard's coefficient of 149 polymorphic AFLP bands from 16 combinations of primers EcoRI and MseI. Analysis of variance of molecular data was performed considering the variation between and within populations of guava from the ten states. The dendrogram showed good definition, with cophenetic coefficient of 0.94. Two major groups were identified: one formed by accessions of guava and other with access to Brazilian guava, including some accessions of guava. Specific groups were observed in the dendrogram only for individuals guava collected in Goias and Roraima. These accessions showed similarity ranging from 28 to 98%, suggesting a high genetic variability of them. The variation among accessions was estimated at 0.16 (ΦST), indicating moderate genetic differentiation among guava populations from the ten states. To increase the genetic variability of the ABG studied, it is suggested to collect more hits in the states of Goiás and Roraima, due to the high degree of similarity among accessions from these states, as well as extensive collections in other Brazilian states. Crosses among the few accessions of guava which are positioned in the group of Brazilian guava were also suggested for the development of interspecific hybrids in the genus Psidium.
Species of the genus Psidium, like guava and Brazilian guava trees, are economically important and have the area of primary genetic diversity in Brazil. It was determined the genetic relationships based on AFLP marker for accessions of Psidium Germplasm Bank (PGB) of Empresa Brasileira de Pesquisa Agropecuária (EMBRAPA) Semiarid to guide improvements and management of genetic resources of the genus. Eighty-eight accessions were analyzed, 64 of guava and 24 of Brazilian guava, collected in ten Brazilian States, adopting for the cluster dendrogram UPGMA, considering the similarity matrix of Jaccard's coefficient of 149 polymorphic AFLP bands from 16 combinations of primers EcoRI and MseI. Analysis of variance of molecular data was performed considering the variation between and within populations of guava from the ten states. The dendrogram showed good definition, with cophenetic coefficient of 0.94. Two major groups were identified: one formed by accessions of guava and other with access to Brazilian guava, including some accessions of guava. Specific groups were observed in the dendrogram only for individuals guava collected in Goias and Roraima. These accessions showed similarity ranging from 28 to 98%, suggesting a high genetic variability of them. The variation among accessions was estimated at 0.16 (ΦST), indicating moderate genetic differentiation among guava populations from the ten states. To increase the genetic variability of the ABG studied, it is suggested to collect more hits in the states of Goiás and Roraima, due to the high degree of similarity among accessions from these states, as well as extensive collections in other Brazilian states. Crosses among the few accessions of guava which are positioned in the group of Brazilian guava were also suggested for the development of interspecific hybrids in the genus Psidium.
Descrição
Palavras-chave
Psidium, banco ativo de germoplasma, melhoramento vegetal, AMOVA, Psidium, germplasm collection, plant breeding, AMOVA
Como citar
Revista Brasileira de Fruticultura. Sociedade Brasileira de Fruticultura, v. 33, n. 3, p. 859-867, 2011.