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dc.contributor.authorNeto Quintal, Amanda Pifano [UNESP]
dc.contributor.authorRibeiro, Erica de Souza [UNESP]
dc.contributor.authorRodrigues, Fernado Pacheco
dc.contributor.authorRocha, Flavia Souza
dc.contributor.authorFloeter-Winter, Lucile Maria
dc.contributor.authorNunes, Caris Maroni [UNESP]
dc.identifier.citationVeterinary Parasitology. Amsterdam: Elsevier B.V., v. 176, n. 2-3, p. 112-119, 2011.
dc.description.abstractLeishmaniasis is kept in nature by the participation of several animal species. This study evaluated the presence of Leishmania spp. in skin samples of free-ranging marsupials Micoureus paraguayanus (n = 95) and Didelphis albiventris (n = 191), captured in Morro do Diabo State Park and in sections of its surrounding forest, in the region of Pontal do Paranapanema, São Paulo State, Brazil. The samples were tested for the presence of kDNA of Leishmania spp. by polymerase chain reaction (PCR) and by real time PCR (qPCR). All samples from D. albiventris tested by PCR were negative for the presence of kDNA of Leishmania spp. However, when tested by qPCR, the positivity was 1.6%. A positivity of 7.4% by PCR and 11.6% by qPCR was observed for M. paraguayanus. Sixty-four per cent (9/14) of positive animals were limited to the same forest fragment. Presence of Leishmania (Leishmania) amazonensis and Leishmania (Viannia) braziliensis was detected in M. paraguayanus samples. While D. albiventris is the most studied marsupial species due to its urban habits, other marsupial species such as M. paraguayanus can be potential reservoirs of Leishmania spp. and should also be studied. (C) 2010 Elsevier B.V. All rights reserved.en
dc.description.sponsorshipCoordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)
dc.description.sponsorshipFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
dc.publisherElsevier B.V.
dc.relation.ispartofVeterinary Parasitology
dc.sourceWeb of Science
dc.subjectPCR and real time PCRen
dc.titleLeishmania spp. in Didelphis albiventris and Micoureus paraguayanus (Didelphimorphia: Didelphidae) of Brazilen
dcterms.rightsHolderElsevier B.V.
dc.contributor.institutionUniversidade Estadual Paulista (Unesp)
dc.contributor.institutionUniversidade de Brasília (UnB)
dc.contributor.institutionUniversidade Federal Rural do Rio de Janeiro (UFRRJ)
dc.contributor.institutionUniversidade de São Paulo (USP)
dc.description.affiliationUniv Estadual Paulista, BR-16050680 São Paulo, Brazil
dc.description.affiliationUniversidade de Brasilia (UnB), Inst Biol Sci, Dept Genet & Morphol, BR-70910900 Brasilia, DF, Brazil
dc.description.affiliationUniv Fed Rural Rio de Janeiro, Inst Forests, Dept Environm Sci, BR-23890000 Seropedica, RJ, Brazil
dc.description.affiliationUniv São Paulo, Fac Biol, Dept Physiol, BR-05508090 São Paulo, Brazil
dc.description.affiliationUnespUniv Estadual Paulista, BR-16050680 São Paulo, Brazil
dc.rights.accessRightsAcesso restrito
dc.description.sponsorshipIdFAPESP: 08/07216-0
unesp.campusUniversidade Estadual Paulista (Unesp), Faculdade de Medicina Veterinária, Araçatubapt
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