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dc.contributor.authorFaraldo, A. C.
dc.contributor.authorGregorio, E. A.
dc.contributor.authorLello, E. [UNESP]
dc.date.accessioned2014-05-20T15:31:20Z
dc.date.available2014-05-20T15:31:20Z
dc.date.issued2008-03-01
dc.identifierhttp://dx.doi.org/10.1016/j.exppara.2007.09.009
dc.identifier.citationExperimental Parasitology. San Diego: Academic Press Inc. Elsevier B.V., v. 118, n. 3, p. 372-377, 2008.
dc.identifier.issn0014-4894
dc.identifier.urihttp://hdl.handle.net/11449/40505
dc.description.abstractInsects manifest effective immune responses that include both cellular and humoral components. Morphological and quantitative aspects of cellular and Immoral cooperation during nodule formation in Chrysomya megacephala hemolymph against Saccharomyces cerevisae yeast cells were demonstrated for the first time. The analyses were performed in non-injected larvae (NIL), saline-injected larvae (SIL) and yeast-injected larvae (YIL). The hemolymph of injected groups was collected 0.5, 1, 2, 4, 12, 24, 36, or 48-h post-injection. Morphological aspects of YIL nodulation were investigated using transmission electron microscopy (TEM). Quantitative analyses consisted of total (THC) and differential hemocyte counts (DHC) in all the groups and total yeast count (TYC) in YIL, which were performed in an improved Neubauer chamber. Nodule formation was initiated at approximately 2-h post-injection. Twelve hours after the injection, TEM revealed the presence of an amorphous membrane, at the same time that circulating hemocyte number decreased significantly contrasting the increase of yeast number. Our results showed the ability of C megacephala hemolymph to perform humoral encapsulation when hemocyte population is insufficient to eliminate the microorganisms, warranting consideration in future investigations on the relative roles played by cellular and humoral elements of innate immunity of this calliphorid. (c) 2007 Elsevier B.V. All rights reserved.en
dc.format.extent372-377
dc.language.isoeng
dc.publisherAcademic Press Inc. Elsevier B.V.
dc.relation.ispartofExperimental Parasitology
dc.sourceWeb of Science
dc.subjectnodule formationen
dc.subjecthumoral encapsulationen
dc.subjecthemolymphen
dc.subjectChrysomya megacephalaen
dc.subjectSaccharomyces cerevisiaeen
dc.titleMorphological and quantitative aspects of nodule formation in hemolymph of the blowfly Chrysomya megacephala (Fabricius, 1794)en
dc.typeArtigo
dcterms.licensehttp://www.elsevier.com/about/open-access/open-access-policies/article-posting-policy
dcterms.rightsHolderAcademic Press Inc. Elsevier B.V.
dc.contributor.institutionUniversidade Federal Rural de Pernambuco (UFRPE)
dc.contributor.institutionUniversidade Estadual Paulista (Unesp)
dc.description.affiliationUniversidade Federal Rural de Pernambuco (UFRPE), Unidade Acad Garanhuns, BR-55292901 Garanhuns, PE, Brazil
dc.description.affiliationUniv Estadual Paulista, Inst Biociencias, Ctr Microscopia Eletron, BR-18618000 São Paulo, Brazil
dc.description.affiliationUniv Estadual Paulista, Inst Biociencias, Dept Morfol, BR-18618000 São Paulo, Brazil
dc.description.affiliationUnespUniv Estadual Paulista, Inst Biociencias, Ctr Microscopia Eletron, BR-18618000 São Paulo, Brazil
dc.description.affiliationUnespUniv Estadual Paulista, Inst Biociencias, Dept Morfol, BR-18618000 São Paulo, Brazil
dc.identifier.doi10.1016/j.exppara.2007.09.009
dc.identifier.wosWOS:000253875400011
dc.rights.accessRightsAcesso restrito
dc.relation.ispartofjcr1.821
dc.relation.ispartofsjr0,635
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