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dc.contributor.authorAndrade, Mariana Carvalho [UNESP]
dc.contributor.authorRibeiro, Ana Paula Dias
dc.contributor.authorDovigo, Lívia Nordi [UNESP]
dc.contributor.authorBrunetti, Iguatemy Lourenço [UNESP]
dc.contributor.authorGiampaolo, Eunice Teresinha [UNESP]
dc.contributor.authorBagnato, Vanderlei Salvador
dc.contributor.authorPavarina, Ana Claudia [UNESP]
dc.date.accessioned2014-05-27T11:28:17Z
dc.date.available2014-05-27T11:28:17Z
dc.date.issued2013-02-01
dc.identifierhttp://dx.doi.org/10.1016/j.archoralbio.2012.10.011
dc.identifier.citationArchives of Oral Biology, v. 58, n. 2, p. 200-210, 2013.
dc.identifier.issn0003-9969
dc.identifier.urihttp://hdl.handle.net/11449/74465
dc.description.abstractObjectives: The aim of this study was to evaluate the effects of pre-irradiation time (PIT) on curcumin (Cur)-mediated photodynamic therapy (PDT) against planktonic and biofilm cultures of reference strains of Candida albicans, Candida glabrata and Candida dubliniensis. Materials and methods: Suspensions and biofilms of Candida species were maintained in contact with different concentrations of Cur for time intervals of 1, 5, 10 and 20 min before irradiation and LED (light emitting diode) activation. Additional samples were treated only with Cur, without illumination, or only with light, without Cur. Control samples received neither light nor Cur. After PDT, suspensions were plated on Sabouraud Dextrose Agar, while biofilm results were obtained using the XTT-salt reduction method. Confocal Laser Scanning Microscopy (CLSM) observations were performed to supply a better understanding of Cur penetration through the biofilms after 5 and 20 min of contact with the cultures. Results: Different PITs showed no statistical differences in Cur-mediated PDT of Candida spp. cell suspensions. There was complete inactivation of the three Candida species with the association of 20.0 μM Cur after 5, 10 and 20 min of PIT. Biofilm cultures showed significant reduction in cell viability after PDT. In general, the three Candida species evaluated in this study suffered higher reductions in cell viability with the association of 40.0 μM Cur and 20 min of PIT. Additionally, CLSM observations showed different intensities of fluorescence emissions after 5 and 20 min of incubation. Conclusion: Photoinactivation of planktonic cultures was not PIT-dependent. PIT-dependence of the biofilm cultures differed among the species evaluated. Also, CLSM observations confirmed the need of higher time intervals for the Cur to penetrate biofilm structures. © 2012 Elsevier Ltd. All rights reserved.en
dc.format.extent200-210
dc.language.isoeng
dc.relation.ispartofArchives of Oral Biology
dc.sourceScopus
dc.subjectBiofilms
dc.subjectCandida
dc.subjectCurcumin
dc.subjectDrug resistance
dc.subjectPhotochemotherapy
dc.subjectCandida albicans
dc.subjectCandida dubliniensis
dc.subjectCandida glabrata
dc.titleEffect of different pre-irradiation times on curcumin-mediated photodynamic therapy against planktonic cultures and biofilms of Candida sppen
dc.typeArtigo
dcterms.licensehttp://www.elsevier.com/about/open-access/open-access-policies/article-posting-policy
dc.contributor.institutionUniversidade Estadual Paulista (UNESP)
dc.contributor.institutionUniversidade de Brasília (UnB)
dc.contributor.institutionUniversidade de São Paulo (USP)
dc.description.affiliationDepartment of Dental Materials and Prosthodontics Araraquara Dental School Universidade Estadual Paulista (UNESP), Araraquara, SP
dc.description.affiliationDepartment of Operative Dentistry University of Brasília - UnB, Brasília, DF
dc.description.affiliationDepartment of Social Dentistry Araraquara Dental School Universidade Estadual Paulista (UNESP), Araraquara, Araraquara, SP
dc.description.affiliationDepartment of Clinical Analysis Universidade Estadual Paulista (UNESP), Araraquara, SP
dc.description.affiliationPhysics Institute of São Carlos University of São Paulo - USP, São Carlos, SP
dc.description.affiliationFaculdade de Odontologia de Araraquara Universidade Estadual Paulista/UNESP, Rua Humaitá, 1680, Centro, CEP: 14801-903 Araraquara, SP
dc.description.affiliationUnespDepartment of Dental Materials and Prosthodontics Araraquara Dental School Universidade Estadual Paulista (UNESP), Araraquara, SP
dc.description.affiliationUnespDepartment of Social Dentistry Araraquara Dental School Universidade Estadual Paulista (UNESP), Araraquara, Araraquara, SP
dc.description.affiliationUnespDepartment of Clinical Analysis Universidade Estadual Paulista (UNESP), Araraquara, SP
dc.description.affiliationUnespFaculdade de Odontologia de Araraquara Universidade Estadual Paulista/UNESP, Rua Humaitá, 1680, Centro, CEP: 14801-903 Araraquara, SP
dc.identifier.doi10.1016/j.archoralbio.2012.10.011
dc.identifier.wosWOS:000314199600011
dc.rights.accessRightsAcesso aberto
dc.identifier.scopus2-s2.0-84874116756
unesp.campusUniversidade Estadual Paulista (UNESP), Faculdade de Ciências Farmacêuticas, Araraquarapt
unesp.campusUniversidade Estadual Paulista (UNESP), Faculdade de Odontologia, Araraquarapt
dc.identifier.file2-s2.0-84874116756.pdf
dc.identifier.lattes5737127334248549
dc.identifier.lattes8867670539105403
dc.identifier.lattes0127570480681342
unesp.author.lattes5737127334248549
unesp.author.lattes8867670539105403
unesp.author.lattes0127570480681342
unesp.author.orcid0000-0002-5435-7609[3]
unesp.author.orcid0000-0003-4927-7599[4]
unesp.author.orcid0000-0002-9231-1994[7]
dc.relation.ispartofjcr2.050
dc.relation.ispartofsjr0,752
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