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dc.contributor.authorSilva, Kathlenn Liezbeth Oliveira [UNESP]
dc.contributor.authorMelo, Larissa Martins [UNESP]
dc.contributor.authorPerosso, Juliana [UNESP]
dc.contributor.authorOliveira, Bruna Brito [UNESP]
dc.contributor.authorSantos, Paulo Sergio Patto dos [UNESP]
dc.contributor.authorEugenio, Flávia de Rezende [UNESP]
dc.contributor.authorLima, Valéria Marçal Felix de [UNESP]
dc.date.accessioned2014-05-27T11:30:50Z
dc.date.available2014-05-27T11:30:50Z
dc.date.issued2013-10-08
dc.identifierhttp://dx.doi.org/10.1016/j.vetpar.2013.07.012
dc.identifier.citationVeterinary Parasitology, v. 197, n. 3-4, p. 470-476, 2013.
dc.identifier.issn0304-4017
dc.identifier.issn1873-2550
dc.identifier.urihttp://hdl.handle.net/11449/76808
dc.description.abstractInfected dogs are urban reservoirs of Leishmania chagasi, which is a causative agent of visceral leishmaniasis (VL). Dogs exhibit immune suppression during the course of this disease, and lymphocyte apoptosis is involved in this process. To investigate apoptosis and the expression levels of FAS-FAS-associated death domain protein (CD95 or APO-1), FASL-FAS ligand protein (CD178), and TRAIL-TNF-related apoptosis-inducing ligand (CD253) receptors in peripheral blood mononuclear cells and spleen leukocytes from 38 symptomatic dogs with moderate VL and 25 healthy dogs were evaluated by flow cytometry. The apoptosis rate of blood and splenic CD4+ and CD8+ cells was higher in infected dogs than in healthy dogs. The expression levels of FAS and FASL in blood and splenic CD4+ cells were lower in infected dogs than in healthy dogs. FAS expression in CD8+ cells was higher in infected dogs than in healthy dogs; in contrast, FASL expression was lower in infected dogs. The expression of the TRAIL receptor increased only in splenic CD8+ cells from infected dogs. The FAS and FAS-L blocking antibodies confirmed the importance of these receptors in apoptosis. Our results enhance the current understanding of the immune response in dogs infected with L. chagasi, facilitating the future development of therapeutic interventions to reduce lymphocyte depletion. © 2013 Elsevier B.V.en
dc.description.sponsorshipFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
dc.format.extent470-476
dc.language.isoeng
dc.relation.ispartofVeterinary Parasitology
dc.sourceScopus
dc.subjectCell death
dc.subjectFAS-associated death domain protein
dc.subjectFASL-FAS ligand protein
dc.subjectLeishmania chagasi
dc.subjectLeishmaniasis
dc.subjectTRAIL (TNF-related apoptosis-inducing ligand)
dc.titleCD95 (FAS) and CD178 (FASL) induce the apoptosis of CD4+ and CD8+ cells isolated from the peripheral blood and spleen of dogs naturally infected with Leishmania sppen
dc.typeArtigo
dcterms.licensehttp://www.elsevier.com/about/open-access/open-access-policies/article-posting-policy
dc.contributor.institutionUniversidade Estadual Paulista (UNESP)
dc.description.affiliationDept. of Clinic Surgery and Animal Reproduction Lab. of Cellular Immunology School of Veter. Sci. of the Sao Paulo State Univ. (Fac. de Med. Veter. da Univ. Est. Paul. Julio de Mesquita Filho), Rua Clóvis Pestana 793, Araçatuba, São Paulo
dc.description.affiliationUnespDept. of Clinic Surgery and Animal Reproduction Lab. of Cellular Immunology School of Veter. Sci. of the Sao Paulo State Univ. (Fac. de Med. Veter. da Univ. Est. Paul. Julio de Mesquita Filho), Rua Clóvis Pestana 793, Araçatuba, São Paulo
dc.identifier.doi10.1016/j.vetpar.2013.07.012
dc.identifier.wosWOS:000328010500008
dc.rights.accessRightsAcesso restrito
dc.description.sponsorshipIdFAPESP: 2011/06214-7
dc.description.sponsorshipIdFAPESP: 2011/02321-3
dc.identifier.scopus2-s2.0-84884969632
unesp.campusUniversidade Estadual Paulista (UNESP), Faculdade de Medicina Veterinária, Araçatubapt
dc.identifier.lattes5331938962896664
dc.identifier.lattes1128699666207940
dc.identifier.lattes4875352337860036
dc.identifier.orcid0000-0002-8535-5569
unesp.author.lattes5331938962896664[5]
unesp.author.lattes1128699666207940
unesp.author.lattes4875352337860036
unesp.author.orcid0000-0002-8535-5569[5]
dc.relation.ispartofjcr2.422
dc.relation.ispartofsjr1,275
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