How can micelle systems be rebuilt by a heating process?
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Data
2012-01-01
Autores
da Silva-Filho, Miguel Adelino
Vieira da Silva Siqueira, Scheyla Daniela
Freire, Larissa Bandeira
de Araujo, Ivonete Batista
Gyselle de Holanda e Silva, Kattya
Medeiros, Aldo da Cunha
Araujo-Filho, Irami
Oliveira, Anselmo Gomes de [UNESP]
Tabosa do Egito, Eryvaldo Socrates
Título da Revista
ISSN da Revista
Título de Volume
Editor
Dove Medical Press Ltd
Resumo
The aim of this work was to evaluate how an aqueous micellar system - containing Amphotericin B (AmB) and sodium deoxycholate (DOC) can be rebuilt after heating treatment. Also, a review of the literature on the physicochemical and biological properties of this new system was conducted. Heated (AmB-DOC-H) and unheated (AmB-DOC) micelles were then diluted at four different concentrations (50 mg.L-1, 5 mg.L-1, 0.5 mg.L-1, and 0.05 mg.L-1) to perform physicochemical studies and a pharmacotoxicity assay, in which two cell models were used for the in vitro experiments: red blood cells (RBC) from human donors and Candida parapsilosis (Cp). While potassium (K+) and hemoglobin leakage from RBC were the parameters used to evaluate acute and chronic toxicity, respectively, the efficacy of AmB-DOC and AmB-DOC-H were assessed by K+ leakage and cell survival rate from Cp. The spectral study revealed a slight change in the AmB-DOC aggregate peak from 327 nm to 323 nm, which is the peak for AmB-DOC-H. Although AmB-DOC and AmB-DOC-H exhibited different behavior for hemoglobin leakage, AmB-DOC produced higher leakage than AmB-DOC-H at high concentrations (from 5 mg.L-1). For K+ leakage, both AmB-DOC and AmB-DOC-H showed a similar profile for both cell models, RBC and Cp (P < 0.05). AmB-DOC-H and AmB-DOC also revealed a similar profile of activity against Cp with an equivalent survival rate. In short, AmB-DOC-H showed much less toxicity than AmB-DOC, but remained as active as AmB-DOC against fungal cells. The results highlight the importance of this new procedure as a simple, inexpensive, and safe way to produce a new kind of micelle system for the treatment of systemic fungal infections.
Descrição
Palavras-chave
micelles, nanotechnology, pre-heated amphotericin B, super-aggregates
Como citar
International Journal of Nanomedicine. Albany: Dove Medical Press Ltd, v. 7, p. 141-150, 2012.