High-performance liquid chromatographic separation and identification of polyphenolic compounds from the infusion of Davilla elliptica St. Hill
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Data
2008-01-01
Autores
Rodrigues, Clenilson M. [UNESP]
Rinaldo, Daniel [UNESP]
Sannomiya, Miriam [UNESP]
Santos, Lourdes Campaner dos [UNESP]
Montoro, Paola
Piacente, Sonia
Pizza, Cosimo
Vilegas, Wagner [UNESP]
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Editor
John Wiley & Sons Ltd
Resumo
The isolation of polyphenolic compounds from an infusion of the Brazilian plant Davilla elliptica (Dilleniaceae), used as tea by virtue of its digestive properties, is described. An improved preparative HPLC method was used in order to isolate pure polyphenols from the complex mixture. Liquid-liquid extraction and solid-phase extraction were employed to minimise the interference of polymeric compounds and to provide an enriched fraction of the compounds of interest. The identification of the isolated compounds was performed using analytical HPLC as well as direct injection electrospray ionisation ion trap tandem mass spectrometry (ESI-IT-MS/MS). The high flavonoid content suggests that D. elliptica may be a promising source of compounds to produce natural phytomedicines. Copyright (C) 2007 John Wiley & Sons, Ltd.
Descrição
Palavras-chave
preparative HPLC, solid-phase extraction, infusion analysis, flavonoids, Davilla elliptica, Dilleniaceac
Como citar
Phytochemical Analysis. Chichester: John Wiley & Sons Ltd, v. 19, n. 1, p. 17-24, 2008.