Desenvolvimento e avaliação em simulador do ecossistema microbiano humano de uma bebida simbiótica à base de extratos aquosos de quinoa (chenopodium quinoa willd) e de soja
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Data
2013-09-23
Autores
Bianchi, Fernanda [UNESP]
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Editor
Universidade Estadual Paulista (Unesp)
Resumo
O conhecimento da população, em relação a uma alimentação mais saudável e nutritiva, tem levado o aumento da procura por alimentos funcionais e que proporcionem uma melhor qualidade de vida. Em função deste interesse, pesquisas que comprovem os benefícios do consumo desses novos produtos tornam-se essenciais. O objetivo do trabalho foi desenvolver uma nova bebida fermentada, potencialmente simbiótica, fermentada com Lactobacillus casei Lc-01 e adicionado de fruto-oligossacarídeo à base de extratos aquosos de quinoa e de soja e avaliar sua ação sob a microbiota intestinal por meio de sistema in vitro. O trabalho foi dividido em duas fazes, na Fase I, cinco formulações com diferentes proporções de extrato aquoso de soja e de quinoa foram testadas. A viabilidade do micro-organismo nas bebidas, assim como os valores de pH e de acidez foram monitorados até o 28º dia de estocagem a 5ºC. Foram também analisadas a composição química dos extratos e das bebidas elaboradas, bem como as propriedades reológicas e sensoriais dos produtos finais. Embora tenha ocorrido um aumento na acidez e um declínio no pH durante os 28 dias de armazenamento das bebidas, o micro-organismo probiótico manteve uma população de 108 UFC.mL-1 para todas as bebidas durante o período experimental. Houve um aumento na viscosidade e na consistência nas bebidas com maior proporção de extrato de quinoa (F1 e F2). A formulação F4 (com 70% extrato de soja- 30% extrato de quinoa) mostrou a menor curva de histerese. As formulações F4 e F5 (com 100% extrato de soja) obtiveram melhor aceitação sensorial e F4 maior intenção de compra. Com relação a composição físico-química, a formulação F3 (com 50% extrato de soja- 50% extrato de quinoa) e F4 mostraram resultados mais próximos aos das bebidas fermentadas à base de soja encontradas na literatura...
The conscience of the population, regarding to a healthier diet, has led to an increase in the demand for functional foods, providing, this way, a better quality of life. Because of this interest, researches demonstrating the benefic consumption of these new products become essential. The aim of this study was to develop a new potentially synbiotic beverage, fermented with Lactobacillus casei Lc-01 and with added fructo-oligosaccharide, based on aqueous extracts of quinoa and soy and to assess its influence on the intestinal microbiota through an in vitro system. The project was divided into two phases, in phase I, five formulations with different proportions of aqueous extract of soy and quinoa were tested. The viability of the microorganism in beverages, as well as the pH and acidity were monitored during 28 days of storage at 5◦C. It was also analyzed the chemical composition of the extracts and beverages as well as the rheological and sensorial properties of the final products. Although there was an increase in acidity and a decline in pH during the 28 days of storage, the probiotic microorganism maintained a population of 108 UFC/mL for all beverage during the experimental period. There was an increase in viscosity and consistency in beverages with higher proportion of quinoa extract (F1 and F2). The formulation F4 (70% soy extract- 30% quinoa extract) showed the lowest hysteresis curve. The formulations F4 and F5 (100% soy extract) obtained the best sensory acceptance, and F4, the higher intention to purchase. Regarding to physical and chemical composition, formulation F3 (with 50% soy extract-50% quinoa extract) and F4 showed the best results compared to similar fermented beverages. Beverage F4 was considered the best beverage developed and, therefore, had its efficacy analyzed from the Simulator Human Microbial Ecosystem (SHIME), phase II of the project... (Complete abstract click electronic access below)
The conscience of the population, regarding to a healthier diet, has led to an increase in the demand for functional foods, providing, this way, a better quality of life. Because of this interest, researches demonstrating the benefic consumption of these new products become essential. The aim of this study was to develop a new potentially synbiotic beverage, fermented with Lactobacillus casei Lc-01 and with added fructo-oligosaccharide, based on aqueous extracts of quinoa and soy and to assess its influence on the intestinal microbiota through an in vitro system. The project was divided into two phases, in phase I, five formulations with different proportions of aqueous extract of soy and quinoa were tested. The viability of the microorganism in beverages, as well as the pH and acidity were monitored during 28 days of storage at 5◦C. It was also analyzed the chemical composition of the extracts and beverages as well as the rheological and sensorial properties of the final products. Although there was an increase in acidity and a decline in pH during the 28 days of storage, the probiotic microorganism maintained a population of 108 UFC/mL for all beverage during the experimental period. There was an increase in viscosity and consistency in beverages with higher proportion of quinoa extract (F1 and F2). The formulation F4 (70% soy extract- 30% quinoa extract) showed the lowest hysteresis curve. The formulations F4 and F5 (100% soy extract) obtained the best sensory acceptance, and F4, the higher intention to purchase. Regarding to physical and chemical composition, formulation F3 (with 50% soy extract-50% quinoa extract) and F4 showed the best results compared to similar fermented beverages. Beverage F4 was considered the best beverage developed and, therefore, had its efficacy analyzed from the Simulator Human Microbial Ecosystem (SHIME), phase II of the project... (Complete abstract click electronic access below)
Descrição
Palavras-chave
Soja, Bebidas fermentadas, Quinoa, Lactobacilo, Lactobacillus
Como citar
BIANCHI, Fernanda. Desenvolvimento e avaliação em simulador do ecossistema microbiano humano de uma bebida simbiótica à base de extratos aquosos de quinoa (chenopodium quinoa willd) e de soja. 2013. 122 f. Dissertação (mestrado) - Universidade Estadual Paulista Júlio de Mesquita Filho, Faculdade de Ciências Farmacêuticas, 2013.