Investigação das propriedades antimicrobianas de frações obtidas a partir dos extratos hidroalcoólico e etanólico de Croton doctoris S. Moore
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Data
2012-07-17
Autores
Borges, Aline Chiodi [UNESP]
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Universidade Estadual Paulista (Unesp)
Resumo
Na última década, tratamentos com plantas medicinais se expandiram pelo mundo e ganharam popularidade. O objetivo desse trabalho foi avaliar o efeito antimicrobiano de frações de extratos de Croton doctoris S. Moore sobre micro-organismos envolvidos na etiologia cárie dentária e identificar substâncias com efeito antimicrobiano dentro das frações mais ativas. Extratos foram obtidos por maceração do pó das folhas em etanol 70º ou etanol 99,5º (1 g / 20 ml de solvente; 72h). Em seguida, frações foram obtidas por extração líquido-líquido dos extratos com diferentes solventes. As frações mais ativas foram selecionadas através do teste de difusão em ágar utilizando cepas de referência (Actinomyces naeslundii ATCC 19039, Lactobacillus acidophilus ATCC 4356, Streptococcus gordonii ATCC 10558, Streptococcus mitis ATCC 9811, Streptococcus mutans ATCC 35688, Streptococcus sanguinis ATCC 10556, Streptococcus sobrinus ATCC 33478). As concentrações inibitórias e bactericidas mínimas (CIM e CBM) das frações mais ativas foram determinadas para as cepas de referência e isolados clínicos de dentina cariada de estreptococos do grupo mutans e lactobacilos. O efeito sobre biofilme em modelo de microcosmo a partir da saliva foi avaliado para as frações mais ativas em concentrações 2, 4 e 10 vezes o valor de CIM. As mesmas frações, em concentração referente a ½ CIM, foram analisadas quanto a capacidade de inibir a adesão de micro-organismos ao esmalte dentário em modelo de microcosmo. A caracterização química das frações ativas foi realizada através de espectometria de massas. A triagem mostrou que as frações diclorometânicas dos extratos hidroalcoólico (HD) e etanólico (ED) e a fração hexânica do extrato hidroalcoólico...
In the past decade, treatment with medicinal plants expanded worldwide and gained popularity. The aim of this study was to evaluate the antimicrobial effect of fractions from extracts of Croton doctoris S. Moore against microorganisms of cariogenic relevance and to identify substances with antimicrobial action in the most active fractions. Crude extracts was obtained by maceration of powdered leaves in 70% ethanol or 99.5% ethanol (1 g / 20 ml, 72 h).The fractions were obtained by liquid-liquid extraction with different solvents. The most active fractions were selected by agar well diffusion test against reference strains (Actinomyces naeslundii ATCC 19039, Lactobacillus acidophilus ATCC 4356, Streptococcus gordonii ATCC 10558, Streptococcus mitis ATCC 9811, Streptococcus mutans ATCC 35688, Streptococcus sanguinis ATCC 10556, Streptococcus sobrinus ATCC 33478). Minimum Inhibitory Concentration (MIC) and Minimum Bactericidal Concentration (MBC) of most active fractions were evaluated against reference strains and clinical isolates of lactobacilus and mutans streptococci. The activity against microcosm biofilm obtained from human saliva was evaluated for the most active fractions at 2, 4 and 10 times MIC. These fractions (½ MIC) were also tested for inhibition of microorganism adhesion to enamel. Chemical characterization of most active fractions was made using mass spectrometry. The screening showed that dichoromethanic fractions of hydroalcoholic and ethanolic extracts (HD and ED) and hexanic fration of hydroalcoholic extract (HH) had antimicrobial activity. The MIC and MBC values for reference strains ranged from 0.1 to 3.1 mg/ml and 0.1 to >50 mg/ml. HD and ED fractions showed the best values. For lactobacilli clinical isolates MIC and MBC ranged from 0.1 to 25 mg/ml and 0.2 to >50 mg/ml... (Complete abstract click electronic access below)
In the past decade, treatment with medicinal plants expanded worldwide and gained popularity. The aim of this study was to evaluate the antimicrobial effect of fractions from extracts of Croton doctoris S. Moore against microorganisms of cariogenic relevance and to identify substances with antimicrobial action in the most active fractions. Crude extracts was obtained by maceration of powdered leaves in 70% ethanol or 99.5% ethanol (1 g / 20 ml, 72 h).The fractions were obtained by liquid-liquid extraction with different solvents. The most active fractions were selected by agar well diffusion test against reference strains (Actinomyces naeslundii ATCC 19039, Lactobacillus acidophilus ATCC 4356, Streptococcus gordonii ATCC 10558, Streptococcus mitis ATCC 9811, Streptococcus mutans ATCC 35688, Streptococcus sanguinis ATCC 10556, Streptococcus sobrinus ATCC 33478). Minimum Inhibitory Concentration (MIC) and Minimum Bactericidal Concentration (MBC) of most active fractions were evaluated against reference strains and clinical isolates of lactobacilus and mutans streptococci. The activity against microcosm biofilm obtained from human saliva was evaluated for the most active fractions at 2, 4 and 10 times MIC. These fractions (½ MIC) were also tested for inhibition of microorganism adhesion to enamel. Chemical characterization of most active fractions was made using mass spectrometry. The screening showed that dichoromethanic fractions of hydroalcoholic and ethanolic extracts (HD and ED) and hexanic fration of hydroalcoholic extract (HH) had antimicrobial activity. The MIC and MBC values for reference strains ranged from 0.1 to 3.1 mg/ml and 0.1 to >50 mg/ml. HD and ED fractions showed the best values. For lactobacilli clinical isolates MIC and MBC ranged from 0.1 to 25 mg/ml and 0.2 to >50 mg/ml... (Complete abstract click electronic access below)
Descrição
Palavras-chave
Cáries dentárias, Produtos biologicos, Esmalte dentário, Plantas medicinais
Como citar
BORGES, Aline Chiodi. Investigação das propriedades antimicrobianas de frações obtidas a partir dos extratos hidroalcoólico e etanólico de Croton doctoris S. Moore. 2012. 89 f. Dissertação (mestrado) – Universidade Estadual Paulista, Faculdade de Odontologia de São José dos Campos, 2012.