Efeito da concentração pós-ovulatória de progesterona nas taxas de concepção de vacas nelore submetidas a IAFT e novilhas cruzadas submetidas a TETF
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Data
2012-08-24
Autores
Frade, Marcelo Carnelli [UNESP]
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Universidade Estadual Paulista (Unesp)
Resumo
Na IATF e TETF o uso da gonadotrofina coriônica eqüina (eCG) é recomendado com o objetivo de incrementar as concentrações plasmáticas de progesterona (P4) e as taxas de concepção. A hipótese do presente estudo é que em fêmeas tratadas com eCG maiores concentrações de P4 são observadas no D5 e D7 (D0 = dia da IATF) em fêmeas prenhes após à IATF e no D5 e D7 (D0 = dia esperado para o estro) em receptoras prenhes submetidas à TETF. No Experimento 1, fêmeas bovinas tratadas ou não com 300UI de eCG e submetidas à IATF, tiveram as concentrações de P4 mensuradas nos dias 0, 3, 5, 7 e 10 após a IATF. No Experimento 2, todas as fêmeas foram tratadas com 300UI de eCG e submetidas à IATF, sendo mensuradas as concentrações de P4 nos dias 3, 5, 7, 12 e 17 após a IATF. No Experimento 3, receptoras tratadas com 300UI de eCG, foram submetidas à TETF, após a observação da presença ou não de estro e as concentrações de P4 foram avaliadas nos dias 5 e 7, após o dia esperado do estro. A dosagem de P4 foi realizada pela técnica de radioimunoensaio e o diagnóstico de gestação através da ultrassonografia. No Experimento 1, observou-se que animais tratados com eCG apresentaram maior concentração de P4 nos dias 0, 7 e 10. No Experimento 2, não houve diferença significativa na concentração de P4 nos dias 3, 7 e 12, entre os animais prenhes e não prenhes, entretanto no dia 17 os animais não prenhes tiveram menor concentração de P4. No Experimento 3, maiores concentrações de P4 no D7 foram observadas em animais que não apresentaram estro e maiores concentrações de P4 foram detectadas em animais prenhes no D7. Conclui-se que em fêmeas tratadas com eCG maiores concentrações de P4 não foram observadas no D5 e D7 (D0 = dia da IATF)...
In FTET and FTAI the use of eCG is recommended in order to increase the synthesis of P4 and the conception rates. The hypothesis of this study is that females treated with eCG higher concentrations of P4 are observed on the D5 and D7 (D0 = day of FTAI) in pregnant females after the FTAI and on the D5 and D7 (D0 = day of expected estrus) in pregnant recipients submitted to FTET. In Experiment 1, cows treated or not with 300 IU of eCG and submitted to FTAI had the concentration of P4 measured on days 0, 3, 5, 7 and 10 after FTAI. In Experiment 2, all females were treated with 300 IU of eCG and submitted to FTAI, being measured the concentration of P4 on days 3, 5, 7, 12 and 17 after FTAI. In Experiment 3, recipients treated with 300 IU of eCG were submitted to FTET, after observation the presence or absence of estrus and measured the concentration of P4 on days 5 and 7 after the expected day of estrus. The P4 assay was performed by radioimmunoassay and pregnancy diagnosis by ultrasonography. In Experiment 1 was observed that animals treated with eCG showed higher concentration of P4 on days 0, 7 and 10. In Experiment 2 there was no significant difference in the concentration of P4 on days 3, 7 and 12 between non-pregnant and pregnant animals, since the day 17 non-pregnant animals had lower concentration of P4. In Experiment 3 highest concentrations of P4 in D7 were observed in animals that did not show estrus and higher concentrations of P4 were detected in pregnant animals on D7. It is concluded that in females treated with eCG higher concentrations of P4 were not observed in D5 and D7 (D0 = day of FTAI) in the pregnant after FTAI, however, the pregnancy rate in FTET embryos produced in vitro was... (Complete abstract click electronic access below)
In FTET and FTAI the use of eCG is recommended in order to increase the synthesis of P4 and the conception rates. The hypothesis of this study is that females treated with eCG higher concentrations of P4 are observed on the D5 and D7 (D0 = day of FTAI) in pregnant females after the FTAI and on the D5 and D7 (D0 = day of expected estrus) in pregnant recipients submitted to FTET. In Experiment 1, cows treated or not with 300 IU of eCG and submitted to FTAI had the concentration of P4 measured on days 0, 3, 5, 7 and 10 after FTAI. In Experiment 2, all females were treated with 300 IU of eCG and submitted to FTAI, being measured the concentration of P4 on days 3, 5, 7, 12 and 17 after FTAI. In Experiment 3, recipients treated with 300 IU of eCG were submitted to FTET, after observation the presence or absence of estrus and measured the concentration of P4 on days 5 and 7 after the expected day of estrus. The P4 assay was performed by radioimmunoassay and pregnancy diagnosis by ultrasonography. In Experiment 1 was observed that animals treated with eCG showed higher concentration of P4 on days 0, 7 and 10. In Experiment 2 there was no significant difference in the concentration of P4 on days 3, 7 and 12 between non-pregnant and pregnant animals, since the day 17 non-pregnant animals had lower concentration of P4. In Experiment 3 highest concentrations of P4 in D7 were observed in animals that did not show estrus and higher concentrations of P4 were detected in pregnant animals on D7. It is concluded that in females treated with eCG higher concentrations of P4 were not observed in D5 and D7 (D0 = day of FTAI) in the pregnant after FTAI, however, the pregnancy rate in FTET embryos produced in vitro was... (Complete abstract click electronic access below)
Descrição
Palavras-chave
Progesterona, Ovarios, Gonadotropinas corionicas, Bovino - Reprodução, Eletrocardiografia, Transferência de embriões, Inseminação artificial, Progesterone
Como citar
FRADE, Marcelo Carnelli. Efeito da concentração pós-ovulatória de progesterona nas taxas de concepção de vacas nelore submetidas a IAFT e novilhas cruzadas submetidas a TETF. 2012. 74 f. Dissertação (mestrado) - Universidade Estadual Paulista Julio de Mesquita Filho, Faculdade de Medicina Veterinária de Araçatuba, 2012.