Glucose, cholesterol, total proteins and insulin-like growth factor type I values in the follicular fluid of female river buffaloes (Bubalus bubalis)

Página do item simplificado
dc.contributor.authorGallego, C.
dc.contributor.authorPardo, Margarita [UNESP]
dc.contributor.authorCamargos, Aline
dc.contributor.authorSousa, D. [UNESP]
dc.contributor.authorLenz, María I.
dc.contributor.authorRamos, A. A. [UNESP]
dc.contributor.authorJorge, A. M.
dc.contributor.authorDa Cruz Ladim, Fernanda [UNESP]
dc.contributor.authorOba, Eunice [UNESP]
dc.contributor.institutionInstituto de Ciencia Animal
dc.contributor.institutionUniversidade Estadual Paulista (UNESP)
dc.contributor.institutionUniversidad Federal de Mato Grosso
dc.date.accessioned2022-04-29T08:49:23Z
dc.date.available2022-04-29T08:49:23Z
dc.date.issued2012-12-01
dc.description.abstractTwenty six Murrah female river buffaloes, between 45 and 70 d post-partum, empty, multiparae, with an average live weight of 675 ± 56 kg, and average body condition of 3.5 points, in a 1 to 5 scale, were used to determine the concentrations of glucose, cholesterol, total protein and insulin-like growth factor type I(IGF-I) in the follicular fluid. The fluid was collected from dominant follicles, with diameters between 8 and 12 mm, by in vivo follicular aspiration. The oestrous cycle stage was not taken into account. The wave of follicular development was synchronized six days prior to the collection. Biochemical analyses of glucose and cholesterol were performed by the enzymatic colorimetric method with the utilization of commercial kits of Glicose (GOD-PAP) and Cholesterol (CHOD-PAP) (Kovalent), respectively. For the determination of total protein, the commercial kit total Protein (Kovalent), method Biuret, was employed. Readings were carried out through absorption spectrophotometry with visible light. Through the radioimmunoanalysis (RIA) technique the concentration of IGF-I was obtained using commercial kits of IRMA IGF-I (IMMUNOTECH). Descriptive statistics was used, by applying the PROC MEANS procedure of the SAS (2009) statistical package. Glucose concentrations (4.0 ± 0.75 mmol/L) and IGF-I (340 ± 129.83 ng/mL) showed higher values in female river buffaloes and dairy cows regarding those reported in other studies. However, cholesterol levels (0.51 ± 0.12 mmol/L) and total proteins (58.4 ± 4.43 g/L) were lower. Results indicate that there is a relationship between the concentration of biochemical indicators, the nutritional aspects, the diameter of the aspired follicles and the productive period.en
dc.description.affiliationInstituto de Ciencia Animal, Apartado Postal 24, San-José de las Lajas, Mayabeque
dc.description.affiliationCampus de Botucatú Universidad Estadual Paulista Julio, Mesquita Filho
dc.description.affiliationCampo Grande Universidad Federal de Mato Grosso, Mato Grosso
dc.description.affiliationUnespCampus de Botucatú Universidad Estadual Paulista Julio, Mesquita Filho
dc.format.extent365-368
dc.identifier.citationCuban Journal of Agricultural Science, v. 46, n. 4, p. 365-368, 2012.
dc.identifier.issn0864-0408
dc.identifier.scopus2-s2.0-84887951528
dc.identifier.urihttp://hdl.handle.net/11449/232166
dc.language.isoeng
dc.relation.ispartofCuban Journal of Agricultural Science
dc.sourceScopus
dc.subjectBiochemical profile
dc.subjectFollicular fluid
dc.subjectPost-partum
dc.titleGlucose, cholesterol, total proteins and insulin-like growth factor type I values in the follicular fluid of female river buffaloes (Bubalus bubalis)en
dc.typeArtigo

Arquivos

Coleções

Artigos - Reprodução Animal e Radiologia Veterinária - FMVZ