Molecular characterization of interferon regulatory factor 1 in Bubalus bubalis

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dc.contributor.authorStafuzza, N. B. [UNESP]
dc.contributor.authorBorges, M. M. [UNESP]
dc.contributor.authorAmaral-Trusty, M. E. J. [UNESP]
dc.contributor.institutionUniversidade Estadual Paulista (Unesp)
dc.date.accessioned2015-12-07T15:33:22Z
dc.date.available2015-12-07T15:33:22Z
dc.date.issued2015
dc.description.abstractInterferon regulatory factor 1 (IRF1) is functionally diverse in the regulation of immune response and is considered to be an important candidate gene for studying disease susceptibility in mammals. In this paper, we characterized the whole sequence of the IRF1 gene in river buffalo (Bubalus bubalis) and compared genomic and the amino acid sequences between different species. The buffalo IRF1 gene was 7099 bp long and organized into 10 exons and nine introns. Its molecular structure showed exactly the same number of exons (10) and introns (nine) in bovids, mice, horses, humans, and chickens. However, rats did not have exon 5, but had the largest exon 4, which suggests that exon 5 was incorporated into exon 4. The coding and the amino acid sequences of the gene showed that identity varied from 73 to 99% at the coding sequence level and from 61 to 100% at the amino acid level when compared with other mammals and chickens. Comparative analysis of the gene sequence between two different buffalo breeds, Murrah and Mediterranean, revealed six potential SNPs that are primarily located in the 5' and 3'UTRs.en
dc.description.affiliationDepartamento de Biologia, Instituto de Biociências, Letras e Ciências Exatas, Universidade Estadual Paulista, São José do Rio Preto, SP, Brasil.
dc.description.affiliationDepartamento de Biologia, Instituto de Biociências, Letras e Ciências Exatas, Universidade Estadual Paulista, São José do Rio Preto, SP, Brasil eamaral@ibilce.unesp.br.
dc.description.affiliationUnespDepartamento de Biologia, Instituto de Biociências, Letras e Ciências Exatas, Universidade Estadual Paulista, São José do Rio Preto, SP, Brasil.
dc.description.affiliationUnespDepartamento de Biologia, Instituto de Biociências, Letras e Ciências Exatas, Universidade Estadual Paulista, São José do Rio Preto, SP, Brasil eamaral@ibilce.unesp.br.
dc.format.extent10919-10928
dc.identifierhttp://dx.doi.org/10.4238/2015.September.21.2
dc.identifier.citationGenetics And Molecular Research : Gmr, v. 14, n. 3, p. 10919-10928, 2015.
dc.identifier.doi10.4238/2015.September.21.2
dc.identifier.issn1676-5680
dc.identifier.pubmed26400319
dc.identifier.urihttp://hdl.handle.net/11449/131279
dc.language.isoeng
dc.publisherGenetics And Molecular Research : Gmr
dc.relation.ispartofGenetics And Molecular Research : Gmr
dc.relation.ispartofsjr0,439
dc.rights.accessRightsAcesso restrito
dc.sourcePubMed
dc.titleMolecular characterization of interferon regulatory factor 1 in Bubalus bubalisen
dc.typeArtigo
unesp.campusUniversidade Estadual Paulista (Unesp), Instituto de Biociências Letras e Ciências Exatas, São José do Rio Pretopt

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