Molecular identification and antimicrobial resistance pattern of Nocardia isolated from 14 diseased dogs and cats

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dc.contributor.authorCondas, Larissa Anuska Zeni [UNESP]
dc.contributor.authorde Farias, Marconi Rodrigues
dc.contributor.authorSiqueira, Amanda Keller [UNESP]
dc.contributor.authorSalerno, Tatiana [UNESP]
dc.contributor.authorChi, Kung Darh
dc.contributor.authorWerner, Juliana
dc.contributor.authorde Vargas, Agueda Castagna
dc.contributor.authorBond, Guilherme Borges
dc.contributor.authorGonoi, Tohru
dc.contributor.authorMatsuzawa, Tetsuhiro
dc.contributor.authorRibeiro, Márcio Garcia [UNESP]
dc.contributor.institutionUniBrasil Centro Universitário
dc.contributor.institutionUniversidade Estadual Paulista (UNESP)
dc.contributor.institutionPontifícia Universidade Católica do Paraná
dc.contributor.institutionLaboratório de Histopatologia Veterinária Werner & Werner
dc.contributor.institutionUniversidade Federal de Santa Maria
dc.contributor.institutionChiba University
dc.contributor.institutionUniversity of Nagasaki
dc.date.accessioned2023-07-29T13:52:10Z
dc.date.available2023-07-29T13:52:10Z
dc.date.issued2023-06-01
dc.description.abstractNocardia are ubiquitous, saprophytic and opportunistic bacteria. They cause a set of pyogenic clinical infections in animals and humans, particularly immunocompromised patients, mostly affecting the skin and respiratory tract, with refractoriness to conventional therapy. The most descriptions of nocardial infections in companion animals involve case reports, and there are scarce case series studies focused on canine and feline nocardiosis in which diagnosis has been based on molecular techniques. We investigated epidemiological aspects, clinical findings, in vitro susceptibility profile, and molecular identification of Nocardia using PCR-based method targeted 16S rRNA gene in twelve dogs and two cats. Among dogs were observed cutaneous lesions (8/12 = 67%), pneumonia (3/12 = 25%), and encephalitis (2/12 = 17%), whereas cats developed cutaneous lesions and osteomyelitis. Nocardia and canine morbillivirus coinfection was described in six dogs (6/12 = 50%). A high mortality rate (6/8 = 75%) was seen among dogs. Three dogs (3/4 = 75%) and one cat (1/2 = 50%) with systemic signs (pneumonia, encephalitis, osteomyelitis), and 83% (5/6) of dogs with a history of concomitant morbillivirus infection died. N. nova (5/12 = 42%), N. cyriacigeorgica (3/12 = 25%), N. farcinica (2/12 = 17%), N. veterana (1/12 = 8%), and N. asteroides (1/12 = 8%) species were identified in dogs, whereas N. africana and N. veterana in cats. Among the isolates from dogs, cefuroxime (12/12 = 100%), amikacin (10/12 = 83%), gentamycin (10/12 = 83%), and imipenem (10/12 = 83%) were the most effective antimicrobials, whereas cefuroxime, cephalexin, amoxicillin/clavulanic acid, imipenem, and gentamycin were efficient against isolates from cats. Multidrug resistance was observed in 36% (5/14) of isolates. We describe a variety of Nocardia species infecting dogs and cats, multidrug-resistant ones, and a high mortality rate, highlighting a poor prognosis of nocardiosis in companion animals, particularly among animals systemically compromised or coinfected by canine morbillivirus. Our study contributes to species identification, in vitro antimicrobial susceptibility profile, clinical-epidemiological aspects, and outcome of natural Nocardia-acquired infections in dogs and cats.en
dc.description.affiliationFaculdade de Medicina Veterinária UniBrasil Centro Universitário, PR
dc.description.affiliationFaculdade de Medicina Veterinária e Zootecnia Departamento de Produção Animal e Medicina Veterinária Preventiva UNESP, SP
dc.description.affiliationFaculdade de Medicina Veterinária Pontifícia Universidade Católica do Paraná, PR
dc.description.affiliationLaboratório de Histopatologia Veterinária Werner & Werner
dc.description.affiliationFaculdade de Medicina Veterinária Universidade Federal de Santa Maria, RS
dc.description.affiliationMedical Mycology Research Center Chiba University
dc.description.affiliationUniversity of Nagasaki
dc.description.affiliationUnespFaculdade de Medicina Veterinária e Zootecnia Departamento de Produção Animal e Medicina Veterinária Preventiva UNESP, SP
dc.description.sponsorshipConselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)
dc.description.sponsorshipIdCNPq: 310345/2020-0
dc.format.extent1287-1294
dc.identifierhttp://dx.doi.org/10.1007/s42770-023-00968-6
dc.identifier.citationBrazilian Journal of Microbiology, v. 54, n. 2, p. 1287-1294, 2023.
dc.identifier.doi10.1007/s42770-023-00968-6
dc.identifier.issn1678-4405
dc.identifier.issn1517-8382
dc.identifier.scopus2-s2.0-85153350634
dc.identifier.urihttp://hdl.handle.net/11449/248733
dc.language.isoeng
dc.relation.ispartofBrazilian Journal of Microbiology
dc.sourceScopus
dc.subjectAntimicrobial profile
dc.subjectCanine and feline nocardiosis
dc.subjectComorbidity
dc.subjectMolecular identification
dc.titleMolecular identification and antimicrobial resistance pattern of Nocardia isolated from 14 diseased dogs and catsen
dc.typeArtigo
unesp.author.orcid0000-0001-5064-9412[1]
unesp.author.orcid0000-0002-1419-8332[3]
unesp.author.orcid0000-0002-9637-4028[7]
unesp.author.orcid0000-0003-3655-7911[9]
unesp.author.orcid0000-0003-2682-9389[11]

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Artigos - Produção Animal - FMVZ