Validação de radioimunoensaio para quantificação de leptina plasmática bovina

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dc.contributor.authorBeltran, Maria Paula [UNESP]
dc.contributor.authorNogueira, Guilherme de Paula
dc.contributor.institutionMédica Veterinária
dc.contributor.institutionUniversidade Estadual Paulista (Unesp)
dc.date.accessioned2014-05-27T11:25:24Z
dc.date.available2014-05-27T11:25:24Z
dc.date.issued2010-12-01
dc.description.abstractDue necessity of better understanding leptin and reproduction relations, a specific radioimmunoassay (RIA) to bovine leptin was avalidated. First, an antibody production protocol was developed using recombinant equine leptin inoculated in a rabbit, that results in 28,05% of maximum binding (MB) 105 days after the protocol beginning. The tests of validations verified parallelism between standard curve and dilutions of high and low controls (P < 0,01). Antibody against equine leptin showed specificity to bovine leptin (P < 0,01). The recuperation tax of bovine leptin by antibody against recombinant equine leptin was from 98,4 to 101,6% (P < 0, 01). When the samples were stored in ambient temperature or refrigerated to 4°C, ligation stability was verified (P > 0,2), however, temperatures above 37°C impaired the bovine leptin recuperation. The use of assay buffer with or without bovine plasma did not show any difference (P > 0,3). These results showed that the antibody produced in rabbit against equine leptin were able to detect plasmatic bovine leptin, and that the RIA to bovine leptin quantification had adequate characteristics to the development of a valid assay.en
dc.description.affiliationMédica Veterinária
dc.description.affiliationLaboratório de Fisiologia e Endocrinologia Animal Universidade Estadual Paulista, Araçatuba-SP
dc.description.affiliationUnespLaboratório de Fisiologia e Endocrinologia Animal Universidade Estadual Paulista, Araçatuba-SP
dc.format.extent5-12
dc.identifierhttp://www.revistas.usp.br/bjvras/article/view/26843
dc.identifier.citationBrazilian Journal of Veterinary Research and Animal Science, v. 47, n. 1, p. 5-12, 2010.
dc.identifier.file2-s2.0-78649427762.pdf
dc.identifier.issn1413-9596
dc.identifier.issn1678-4456
dc.identifier.scopus2-s2.0-78649427762
dc.identifier.urihttp://hdl.handle.net/11449/72186
dc.language.isopor
dc.relation.ispartofBrazilian Journal of Veterinary Research and Animal Science
dc.relation.ispartofsjr0,225
dc.relation.ispartofsjr0,225
dc.rights.accessRightsAcesso aberto
dc.sourceScopus
dc.subjectAntibody
dc.subjectLeptin
dc.subjectRadioimmunoassay
dc.subjectValidation
dc.subjectBovinae
dc.subjectEquidae
dc.subjectOryctolagus cuniculus
dc.titleValidação de radioimunoensaio para quantificação de leptina plasmática bovinapt
dc.title.alternativeImunoradioassay validation to plasmatic bovine leptin quantificationen
dc.typeArtigo
dcterms.licensehttp://www.scielo.br/revistas/bjvras/paboutj.htm
unesp.campusUniversidade Estadual Paulista (Unesp), Faculdade de Odontologia, Araçatubapt

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