Nuclear phenotypes and morphometry of human secretory prostate cells: A comparative study of benign and malignant lesions in Brazilian patients

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dc.contributor.authorTaboga, Sebastião R.
dc.contributor.authorSantos, Adriana B. dos [UNESP]
dc.contributor.authorGonzatti, Adriana G. R.
dc.contributor.authorVidal, Benedicto C.
dc.contributor.authorMello, Maria Luiza S.
dc.contributor.institutionUniversidade Estadual de Campinas (UNICAMP)
dc.contributor.institutionUniversidade Estadual Paulista (Unesp)
dc.contributor.institutionUniversidade de São Paulo (USP)
dc.date.accessioned2014-05-27T11:20:40Z
dc.date.available2014-05-27T11:20:40Z
dc.date.issued2003-07-01
dc.description.abstractProstatic lesions in Brazilian patients with benign prostatic hyperplasia (BPH, 26 cases) or adenocarcinoma (AC, 25 cases) were compared by qualitative microscopy and morphometric analysis. In 12 cases of BPH, prostate regions with no histological alterations were considered as controls (Ct). Archival material consisted of formalin-fixed, paraffin-embedded specimens obtained from prostatic transurethral resection and radical prostatectomy. Haematoxylin/eosin (HE)-stained sections were used to estimate the nuclear areas, perimeters and form factor values. HE-stained sections from AC specimens were also used for Gleason grading. BPH, AC and Ct could be discriminated by their nuclear areas and nuclear perimeters, but not by the nuclear form factor parameter. No significant differences were found when the AC data were compared using the combined version or the predominant grade version of the Gleason score (p = 0.8380 for nuclear area; p = 0.6076 for nuclear perimeter; p = 0.9202 for nuclear form factor; n = 200 nuclei per patient). This finding indicates that there is extensive heterogeneity in the size and shape of the nucleus in AC cells. These results also show that although the nuclear morphometry served to discriminate BPH and AC from each other and from Ct, it was not sufficient to correlate AC lesions with their respective Gleason scores in the human population analyzed.en
dc.description.affiliationDepartment of Cell Biology Institute of Biology UNICAMP, 13084-971 Campinas, SP
dc.description.affiliationDepartment of Biology, Rua Cristovão Colombo, 2265 - Jardim Nazareth, Sao José do Rio Preto, São Paulo
dc.description.affiliationDepartment of Computation Science and Statistics Institute of Bioscience, Humanities and Exact Sciences UNESP, 15054-000 Sao José do Rio Preto, SP
dc.description.affiliationDepartment of Pathology Faculty of Medical Sciences UNICAMP, 13084-971 Campinas, SP
dc.description.affiliationUnespDepartment of Computation Science and Statistics Institute of Bioscience, Humanities and Exact Sciences UNESP, 15054-000 Sao José do Rio Preto, SP
dc.format.extent315-322
dc.identifierhttp://dx.doi.org/10.1080/00087114.2003.10589340
dc.identifier.citationCaryologia, v. 56, n. 3, p. 315-322, 2003.
dc.identifier.doi10.1080/00087114.2003.10589340
dc.identifier.issn0008-7114
dc.identifier.lattes7055362276236996
dc.identifier.orcid0000-0002-0970-4288
dc.identifier.orcid0000-0003-4076-2475
dc.identifier.scopus2-s2.0-77949752594
dc.identifier.urihttp://hdl.handle.net/11449/67331
dc.identifier.wosWOS:000186419400010
dc.language.isoeng
dc.relation.ispartofCaryologia
dc.relation.ispartofjcr0.608
dc.relation.ispartofsjr0,281
dc.rights.accessRightsAcesso restrito
dc.sourceScopus
dc.subjectBenign lesions
dc.subjectBrazilian patients
dc.subjectHuman prostate
dc.subjectMalignant lesions
dc.subjectNuclear morphometry
dc.titleNuclear phenotypes and morphometry of human secretory prostate cells: A comparative study of benign and malignant lesions in Brazilian patientsen
dc.typeArtigo
dcterms.licensehttp://journalauthors.tandf.co.uk/permissions/reusingOwnWork.asp
unesp.author.lattes7055362276236996[2]
unesp.author.orcid0000-0002-0970-4288[1]
unesp.author.orcid0000-0003-4076-2475[2]
unesp.campusUniversidade Estadual Paulista (Unesp), Instituto de Biociências Letras e Ciências Exatas, São José do Rio Pretopt

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