Low level laser therapy increases angiogenesis in a model of ischemic skin flap in rats mediated by VEGF, HIF-1α and MMP-2

Página do item simplificado
dc.contributor.authorCury, Vivian
dc.contributor.authorMoretti, Ana Iochabel Soares
dc.contributor.authorAssis, Lívia
dc.contributor.authorBossini, Paulo
dc.contributor.authorDe Souza Crusca, Jaqueline
dc.contributor.authorBenatti Neto, Carlos[UNESP]
dc.contributor.authorFangel, Renan
dc.contributor.authorDe Souza, Heraldo Possolo
dc.contributor.authorHamblin, Michael R.
dc.contributor.authorParizotto, Nivaldo Antonio
dc.contributor.institutionUniversidade Federal de São Carlos (UFSCar)
dc.contributor.institutionUniversidade de São Paulo (USP)
dc.contributor.institutionInstituto de Assistência Médica ao Servidor Público Estadual-IAMSPE
dc.contributor.institutionUniversidade Estadual Paulista (Unesp)
dc.contributor.institutionMassachusetts General Hospital
dc.contributor.institutionHarvard Medical School
dc.contributor.institutionHarvard-MIT Division of Health Sciences and Technology
dc.date.accessioned2014-05-27T11:29:57Z
dc.date.available2014-05-27T11:29:57Z
dc.date.issued2013-07-11
dc.description.abstractIt is known that low level laser therapy is able to improve skin flap viability by increasing angiogenesis. However, the mechanism for new blood vessel formation is not completely understood. Here, we investigated the effects of 660 nm and 780 nm lasers at fluences of 30 and 40 J/cm2 on three important mediators activated during angiogenesis. Sixty male Wistar rats were used and randomly divided into five groups with twelve animals each. Groups were distributed as follows: skin flap surgery non-irradiated group as a control; skin flap surgery irradiated with 660 nm laser at a fluence of 30 or 40 J/cm2 and skin flap surgery irradiated with 780 nm laser at a fluence of 30 or 40 J/cm2. The random skin flap was performed measuring 10 × 4 cm, with a plastic sheet interposed between the flap and the donor site. Laser irradiation was performed on 24 points covering the flap and surrounding skin immediately after the surgery and for 7 consecutive days thereafter. Tissues were collected, and the number of vessels, angiogenesis markers (vascular endothelial growth factor, VEGF and hypoxia inducible factor, HIF-1α) and a tissue remodeling marker (matrix metalloproteinase, MMP-2) were analyzed. LLLT increased an angiogenesis, HIF-1α and VEGF expression and decrease MMP-2 activity. These phenomena were dependent on the fluences, and wavelengths used. In this study we showed that LLLT may improve the healing of skin flaps by enhancing the amount of new vessels formed in the tissue. Both 660 nm and 780 nm lasers were able to modulate VEGF secretion, MMP-2 activity and HIF-1α expression in a dose dependent manner. © 2013 Published by Elsevier B.V.en
dc.description.affiliationDepartment of Physiotherapy Federal University of São Carlos, São Carlos, São Paulo
dc.description.affiliationEmergency Medicine Division Faculdade de Medicina da Universidade de São Paulo, São Paulo
dc.description.affiliationPost-Graduate Health Sciences Program Instituto de Assistência Médica ao Servidor Público Estadual-IAMSPE, São Paulo, SP
dc.description.affiliationDepartment of Physiology and Pathology Universidade Estadual de São Paulo, Araraquara, São Paulo
dc.description.affiliationWellman Center for Photomedicine Massachusetts General Hospital, Boston, MA 02114
dc.description.affiliationDepartment of Dermatology Harvard Medical School, Boston, MA 02115
dc.description.affiliationHarvard-MIT Division of Health Sciences and Technology, Cambridge, MA 02139
dc.description.affiliationLaboratory of Medical Research Faculdade de Medicina Universidade de Sao Paulo, Av. Dr. Arnaldo, 455 sala 3189, CEP 01246-903 Sao Paulo, SP
dc.description.affiliationUnespDepartment of Physiology and Pathology Universidade Estadual de São Paulo, Araraquara, São Paulo
dc.format.extent164-170
dc.identifierhttp://dx.doi.org/10.1016/j.jphotobiol.2013.06.004
dc.identifier.citationJournal of Photochemistry and Photobiology B: Biology, v. 125, p. 164-170.
dc.identifier.doi10.1016/j.jphotobiol.2013.06.004
dc.identifier.issn1011-1344
dc.identifier.issn1873-2682
dc.identifier.scopus2-s2.0-84879803797
dc.identifier.urihttp://hdl.handle.net/11449/75950
dc.language.isoeng
dc.relation.ispartofJournal of Photochemistry and Photobiology B: Biology
dc.relation.ispartofjcr3.165
dc.relation.ispartofsjr0,698
dc.rights.accessRightsAcesso restrito
dc.sourceScopus
dc.subjectAngiogenesis
dc.subjectHIF1-α
dc.subjectHypoxia
dc.subjectLLLT
dc.subjectMMP-2
dc.subjectVEGF
dc.titleLow level laser therapy increases angiogenesis in a model of ischemic skin flap in rats mediated by VEGF, HIF-1α and MMP-2en
dc.typeArtigo
dcterms.licensehttp://www.elsevier.com/about/open-access/open-access-policies/article-posting-policy
unesp.author.lattes5598845588411528[6]
unesp.campusUniversidade Estadual Paulista (Unesp), Faculdade de Odontologia, Araraquarapt

Arquivos

Coleções

Artigos - Fisiologia e Patologia - FOAR