A Novel Apilic Antivenom to Treat Massive, Africanized Honeybee Attacks: A Preclinical Study from the Lethality to Some Biochemical and Pharmacological Activities Neutralization

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dc.contributor.authorTeixeira-Cruz, Jhonatha Mota
dc.contributor.authorStrauch, Marcelo Abrahão
dc.contributor.authorMonteiro-Machado, Marcos
dc.contributor.authorTavares-Henriques, Matheus Silva
dc.contributor.authorde Moraes, João Alfredo
dc.contributor.authorRibeiro da Cunha, Luís Eduardo
dc.contributor.authorFerreira, Rui Seabra [UNESP]
dc.contributor.authorBarraviera, Benedito [UNESP]
dc.contributor.authorQuintas, Luis Eduardo M
dc.contributor.authorMelo, Paulo A.
dc.contributor.institutionFederal University of Rio de Janeiro
dc.contributor.institutionScientific Board
dc.contributor.institutionUniversidade Estadual Paulista (Unesp)
dc.date.accessioned2021-06-25T10:50:55Z
dc.date.available2021-06-25T10:50:55Z
dc.date.issued2021-01-05
dc.description.abstractMassive, Africanized honeybee attacks have increased in Brazil over the years. Humans and animals present local and systemic effects after envenomation, and there is no specific treatment for this potentially lethal event. This study evaluated the ability of a new Apilic antivenom, which is composed of F(ab')2 fraction of specific immunoglobulins in heterologous and hyperimmune equine serum, to neutralize A. mellifera venom and melittin, in vitro and in vivo, in mice. Animal experiments were performed in according with local ethics committee license (UFRJ protocol no. DFBCICB072-04/16). Venom dose-dependent lethality was diminished with 0.25-0.5 μL of intravenous Apilic antivenom/μg honeybee venom. In vivo injection of 0.1-1 μg/g bee venom induced myotoxicity, hemoconcentration, paw edema, and increase of vascular permeability which were antagonized by Apilic antivenom. Cytotoxicity, assessed in renal LLC-PK1 cells and challenged with 10 μg/mL honeybee venom or melittin, was neutralized by preincubation with Apilic antivenom, as well the hemolytic activity. Apilic antivenom inhibited phospholipase and hyaluronidase enzymatic activities. In flow cytometry experiments, Apilic antivenom neutralized reduction of cell viability due to necrosis by honeybee venom or melittin. These results showed that this antivenom is effective inhibitor of honeybee venom actions. Thus, this next generation of Apilic antivenom emerges as a new promising immunobiological product for the treatment of massive, Africanized honeybee attacks.en
dc.description.affiliationGraduate Program in Pharmacology and Medicinal Chemistry Institute of Biomedical Sciences Federal University of Rio de Janeiro
dc.description.affiliationScientific Board
dc.description.affiliationCenter for the Study of Venoms and Venomous Animals (CEVAP) São Paulo State University (UNESP)
dc.description.affiliationUnespCenter for the Study of Venoms and Venomous Animals (CEVAP) São Paulo State University (UNESP)
dc.description.sponsorshipCoordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)
dc.description.sponsorshipConselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)
dc.description.sponsorshipFundação de Amparo à Pesquisa do Estado do Rio de Janeiro (FAPERJ)
dc.description.sponsorshipIdCAPES: 23038.006285/2011-21
dc.description.sponsorshipIdCNPq: 401170/2013-6
dc.description.sponsorshipIdCNPq: 409436/2016-0
dc.description.sponsorshipIdFAPERJ: e-26/200.006/2018
dc.description.sponsorshipIdFAPERJ: E_02/2017
dc.identifierhttp://dx.doi.org/10.3390/toxins13010030
dc.identifier.citationToxins, v. 13, n. 1, 2021.
dc.identifier.doi10.3390/toxins13010030
dc.identifier.issn2072-6651
dc.identifier.scopus2-s2.0-85100229954
dc.identifier.urihttp://hdl.handle.net/11449/207217
dc.language.isoeng
dc.relation.ispartofToxins
dc.sourceScopus
dc.subjectApilic antivenom
dc.subjectApis mellifera
dc.subjectenvenomation
dc.subjecthoneybee venom
dc.subjectmelittin
dc.subjectpreclinical study
dc.titleA Novel Apilic Antivenom to Treat Massive, Africanized Honeybee Attacks: A Preclinical Study from the Lethality to Some Biochemical and Pharmacological Activities Neutralizationen
dc.typeArtigo
unesp.author.orcid0000-0002-3167-0723[1]
unesp.author.orcid0000-0002-1657-1356[3]
unesp.author.orcid0000-0003-0197-9778[4]
unesp.author.orcid0000-0002-8563-6432[5]
unesp.author.orcid0000-0001-6952-0512[7]
unesp.author.orcid0000-0002-0767-1240[9]

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Botucatu - CEVAP - Centro de Estudos de Venenos e Animais Peçonhentos