Strength training prior to muscle injury potentiates low-level laser therapy (LLLT)-induced muscle regeneration

Página do item simplificado
dc.contributor.authorMorais, Samuel Rodrigues Lourenço [UNESP]
dc.contributor.authorGoya, Alexandre Ginei [UNESP]
dc.contributor.authorUrias, Úrsula
dc.contributor.authorJannig, Paulo Roberto
dc.contributor.authorBacurau, Aline Villa Nova
dc.contributor.authorMello, Wagner Garcez [UNESP]
dc.contributor.authorFaleiros, Paula Lazilha [UNESP]
dc.contributor.authorOliveira, Sandra Helena Penha [UNESP]
dc.contributor.authorGarcia, Valdir Gouveia [UNESP]
dc.contributor.authorErvolino, Edilson [UNESP]
dc.contributor.authorBrum, Patricia Chakur
dc.contributor.authorDornelles, Rita Cássia Menegati [UNESP]
dc.contributor.institutionUniversidade Estadual Paulista (Unesp)
dc.contributor.institutionLaboratory of Endocrine Physiology and Aging—Department of Basic Sciences
dc.contributor.institutionUniversidade de São Paulo (USP)
dc.date.accessioned2018-12-11T17:23:17Z
dc.date.available2018-12-11T17:23:17Z
dc.date.issued2017-02-01
dc.description.abstractWe evaluated whether strength training (ST) performed prior to skeletal muscle cryolesion would act as a preconditioning, improving skeletal muscle regeneration and responsiveness to low-level laser therapy (LLLT). Wistar rats were randomly assigned into non-exercised (NE), NE plus muscle lesion (NE + LE), NE + LE plus LLLT (NE + LE + LLLT), strength training (ST), ST + LE, and ST + LE + LLLT. The animals performed 10 weeks of ST (climbing ladder; 3× week; 80% overload). Forty-eight hours after the last ST session, tibialis anterior (TA) cryolesion was induced and LLLT (InGaAlP, 660 nm, 0.035 W, 4.9 J/cm2/point, 3 points, spot light 0.028 cm2, 14 J/cm2) initiated and conducted daily for 14 consecutive days. The difference between intergroups was assessed using Student’s t test and intragroups by two-way analysis of variance. Cryolesion induced massive muscle degeneration associated with inflammatory infiltrate. Prior ST improved skeletal regeneration 14-days after cryolesion and potentiated the regenerative response to LLLT. Cryolesion induced increased TNF-α levels in both NE + LE and ST + LE groups. Both isolated ST and LLLT reduced TNF-α to control group levels; however, prior ST potentiated LLLT response. Both isolated ST and LLLT increased IL-10 levels with no additional effect. In contrast, increased TA IL-6 levels were restricted to ST and ST + LE + LLLT groups. TA myogenin mRNA levels were not changed by neither prior ST or ST + LLLT. Both prior ST and LLLT therapies increased MyoD mRNA levels and, interestingly, combined therapies potentiated this response. Myf5 mRNA levels were increased only in ST groups. Taken together, our data provides evidences for prior ST potentiating LLLT efficacy in promoting skeletal muscle regeneration.en
dc.description.affiliationPrograma Multicêntrico de Pós-Graduação em Ciências Fisiológicas—SBFis/UNESP
dc.description.affiliationLaboratory of Endocrine Physiology and Aging—Department of Basic Sciences
dc.description.affiliationSão Paulo State University (UNESP) School of Dentistry
dc.description.affiliationSchool of Physical Education and Sport University of Sao Paulo
dc.description.affiliationGroup of Research and Study on Laser in Dentistry (GEPLO) Division of Periodontics Department of Surgery and Integrated Clinic São Paulo State University (UNESP) School of Dentistry
dc.description.affiliationExperimental Physiopathology—Medical School University of Sao Paulo
dc.description.affiliationUnespPrograma Multicêntrico de Pós-Graduação em Ciências Fisiológicas—SBFis/UNESP
dc.description.affiliationUnespSão Paulo State University (UNESP) School of Dentistry
dc.description.affiliationUnespGroup of Research and Study on Laser in Dentistry (GEPLO) Division of Periodontics Department of Surgery and Integrated Clinic São Paulo State University (UNESP) School of Dentistry
dc.description.sponsorshipFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
dc.description.sponsorshipIdFAPESP: 2013/18907-2
dc.format.extent317-325
dc.identifierhttp://dx.doi.org/10.1007/s10103-016-2116-3
dc.identifier.citationLasers in Medical Science, v. 32, n. 2, p. 317-325, 2017.
dc.identifier.doi10.1007/s10103-016-2116-3
dc.identifier.file2-s2.0-85000885491.pdf
dc.identifier.issn1435-604X
dc.identifier.issn0268-8921
dc.identifier.lattes4408095517346846
dc.identifier.lattes5435902422784889
dc.identifier.orcid0000-0003-4859-0583
dc.identifier.orcid0000-0003-0783-6612
dc.identifier.scopus2-s2.0-85000885491
dc.identifier.urihttp://hdl.handle.net/11449/176963
dc.language.isoeng
dc.relation.ispartofLasers in Medical Science
dc.relation.ispartofsjr0,713
dc.rights.accessRightsAcesso aberto
dc.sourceScopus
dc.subjectLow-level laser therapy
dc.subjectMyf5
dc.subjectMyoD
dc.subjectMyogenin
dc.subjectSkeletal muscle regeneration
dc.subjectStrength training
dc.titleStrength training prior to muscle injury potentiates low-level laser therapy (LLLT)-induced muscle regenerationen
dc.typeArtigo
unesp.author.lattes4408095517346846[10]
unesp.author.lattes5435902422784889[12]
unesp.author.orcid0000-0003-4859-0583[10]
unesp.author.orcid0000-0003-0783-6612[12]

Arquivos

Pacote Original
Agora exibindo 1 - 1 de 1
Imagem de Miniatura
Nome:
2-s2.0-85000885491.pdf
Tamanho:
959.74 KB
Formato:
Adobe Portable Document Format
Descrição:
Baixar

Coleções

Artigos - Patologia - FMB