Interleukin-4 and interleukin-13 inhibit the expression of leukemia inhibitory factor and interleukin-11 in fibroblasts

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dc.contributor.authorSouza, Pedro P. C. [UNESP]
dc.contributor.authorPalmqvist, Py
dc.contributor.authorLundberg, Pernilla
dc.contributor.authorLundgren, Inger
dc.contributor.authorHanstrom, Lennart
dc.contributor.authorSouza, Joao A. C.
dc.contributor.authorConaway, H. Herschel
dc.contributor.authorLerner, Ulf H.
dc.contributor.institutionUmea Univ
dc.contributor.institutionUniversidade Estadual Paulista (Unesp)
dc.contributor.institutionUniv Arkansas Med Sci
dc.contributor.institutionUniv Gothenburg
dc.date.accessioned2014-05-20T13:45:59Z
dc.date.available2014-05-20T13:45:59Z
dc.date.issued2012-01-01
dc.description.abstractCytokines produced by inflammatory or resident mesenchymal cells play important modulatory roles in the pathogenesis of inflammation induced bone loss. In the present study, the effects of IL-4 and IL-13 on the expression of three osteotropic cytokines in the IL-6 family expressed in human gingival fibroblasts were studied. IL-4R alpha and IL-13R alpha 1 mRNA were constitutively expressed in human gingival fibroblasts. The inflammatory cytokines IL-1 beta and TNF-alpha increased expression of IL-5, LIF, and IL-11 mRNA and protein in the gingival fibroblasts. Addition of IL-4 or IL-13 had no effect on IL-6 expression, but significantly inhibited LIF and IL-11 mRNA and protein stimulated by IL-1 beta and TNF-alpha. No involvement of NF-kappa B or STAT1 was observed in the inhibition. STAT6 was phosphorylated at Y641 by treatment with IL-4 and knockdown of STAT6 with siRNA decreased the inhibition of IL-11 and LIF expression by IL-4 in IL-1 beta and TNF-alpha stimulated cells. This study suggests that activation of STAT6 by IL-4 and IL-13, through type 2 IL-4 receptors, inhibits production of IL-11 and LIF stimulated by IL-1 beta and TNF-alpha in human gingival fibroblasts. A negative modulatory role of IL-4 and IL-13 in osteotropic cytokine production could be a mechanism playing an important inhibitory role in inflammation induced periodontitis. (C) 2011 Elsevier Ltd. All rights reserved.en
dc.description.affiliationUmea Univ, Dept Mol Periodontol, SE-90187 Umea, Sweden
dc.description.affiliationSão Paulo State Univ, Araraquara Sch Dent, Dept Physiol & Pathol, Araraquara, Brazil
dc.description.affiliationUniv Arkansas Med Sci, Dept Physiol & Biophys, Little Rock, AR 72205 USA
dc.description.affiliationUniv Gothenburg, Sahlgrenska Acad, Inst Med, Ctr Bone & Arthrit Res, Gothenburg, Sweden
dc.description.affiliationUnespSão Paulo State Univ, Araraquara Sch Dent, Dept Physiol & Pathol, Araraquara, Brazil
dc.description.sponsorshipSwedish Research Council
dc.description.sponsorshipSwedish Dental Society
dc.description.sponsorshipRoyal 80 Year Fund
dc.description.sponsorshipSwedish Rheumatism Association
dc.description.sponsorshipFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
dc.format.extent601-610
dc.identifierhttp://dx.doi.org/10.1016/j.molimm.2011.10.009
dc.identifier.citationMolecular Immunology. Oxford: Pergamon-Elsevier B.V. Ltd, v. 49, n. 4, p. 601-610, 2012.
dc.identifier.doi10.1016/j.molimm.2011.10.009
dc.identifier.issn0161-5890
dc.identifier.urihttp://hdl.handle.net/11449/16233
dc.identifier.wosWOS:000300212100005
dc.language.isoeng
dc.publisherPergamon-Elsevier B.V. Ltd
dc.relation.ispartofMolecular Immunology
dc.relation.ispartofjcr3.188
dc.relation.ispartofsjr1,352
dc.rights.accessRightsAcesso restrito
dc.sourceWeb of Science
dc.subjectInterleukin-4en
dc.subjectInterleukin-6en
dc.subjectGingival fibroblastsen
dc.subjectBone resorptionen
dc.titleInterleukin-4 and interleukin-13 inhibit the expression of leukemia inhibitory factor and interleukin-11 in fibroblastsen
dc.typeArtigo
dcterms.licensehttp://www.elsevier.com/about/open-access/open-access-policies/article-posting-policy
dcterms.rightsHolderPergamon-Elsevier B.V. Ltd
unesp.author.orcid0000-0002-8053-2407[6]
unesp.author.orcid0000-0001-7266-6061[6]
unesp.campusUniversidade Estadual Paulista (Unesp), Faculdade de Odontologia, Araraquarapt

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