The antiepileptic activity of JSTX-3 is mediated by N-methyl-D-aspartate receptors in human hippocampal neurons

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dc.contributor.authorSalamoni, S. D.
dc.contributor.authorda Costa, J. C.
dc.contributor.authorPalma, Mario Sergio [UNESP]
dc.contributor.authorKonno, K.
dc.contributor.authorNihei, K.
dc.contributor.authorAzambuja, N. A.
dc.contributor.authorNeto, E. P.
dc.contributor.authorVenturin, G. T.
dc.contributor.authorTavares, A. A.
dc.contributor.authorde Abreu, D. S.
dc.contributor.authorBreda, R. V.
dc.contributor.institutionPontificia Univ Catolica Rio Grande do Sul
dc.contributor.institutionUniversidade Estadual Paulista (Unesp)
dc.contributor.institutionFdn Res Support State São Paulo
dc.date.accessioned2014-05-20T15:28:29Z
dc.date.available2014-05-20T15:28:29Z
dc.date.issued2005-11-07
dc.description.abstractWe analyzed the effect of the acylpolyaminetoxin JSTX-3 on the epileptogenic discharges induced by perfusion of human hippocampal slices with artificial cerebrospinal fluid lacking Mg2+ or N-methyl-D-aspartate. Hippocampi were surgically removed from patients with refractory medial temporal lobe epilepsy, sliced in the surgical room and taken to the laboratory immersed in normal artificial cerebrospinal fluid. Epileptiform activity was induced by perfusion with Mg2+-free artificial cerebrospinal fluid or by iontophoretically applied N-methyl-D-aspartate and intracellular and field recordings of CAI neurons were performed. The ictal-like discharges induced by Mg2+-free artificial cerebrospinal fluid and N-methyl-D-aspartate were blocked by incubation with JSTX-3. This effect was similar to that obtained with the N-methyl-D-aspartate receptor antagonist DL(-)2-amino-5 phosphonovaleric acid. Our findings suggest that in human hippocampal neurons, the antiepileptic effect of JSTX-3 is mediated by its action on N-methyl-D-aspartate receptor.en
dc.description.affiliationPontificia Univ Catolica Rio Grande do Sul, Neurosci Lab, Biomed Res Inst, BR-90610000 Porto Alegre, RS, Brazil
dc.description.affiliationPontificia Univ Catolica Rio Grande do Sul, Epilepsy Surg Program, BR-90610000 Porto Alegre, RS, Brazil
dc.description.affiliationState Univ São Paulo, Dept Biol, Biosci Inst, Ctr Social Insects Study, São Paulo, Brazil
dc.description.affiliationFdn Res Support State São Paulo, Instituto Butantan, São Paulo, Brazil
dc.description.affiliationFdn Res Support State São Paulo, Ctr Appl Toxicol, Ctr Res Innovat & Diffus, São Paulo, Brazil
dc.description.affiliationUnespState Univ São Paulo, Dept Biol, Biosci Inst, Ctr Social Insects Study, São Paulo, Brazil
dc.format.extent1869-1873
dc.identifierhttp://dx.doi.org/10.1097/01.wnr.0000185012.98821.2b
dc.identifier.citationNeuroreport. Philadelphia: Lippincott Williams & Wilkins, v. 16, n. 16, p. 1869-1873, 2005.
dc.identifier.doi10.1097/01.wnr.0000185012.98821.2b
dc.identifier.issn0959-4965
dc.identifier.lattes2901888624506535
dc.identifier.urihttp://hdl.handle.net/11449/38285
dc.identifier.wosWOS:000233277700030
dc.language.isoeng
dc.publisherLippincott Williams & Wilkins
dc.relation.ispartofNeuroreport
dc.relation.ispartofjcr1.266
dc.relation.ispartofsjr0,644
dc.rights.accessRightsAcesso restrito
dc.sourceWeb of Science
dc.subjectepilepsypt
dc.subjecthippocampuspt
dc.subjectJSTX-3pt
dc.subjectN-methyl-D-aspartate receptorspt
dc.titleThe antiepileptic activity of JSTX-3 is mediated by N-methyl-D-aspartate receptors in human hippocampal neuronsen
dc.typeArtigo
dcterms.licensehttp://journals.lww.com/_layouts/oaks.journals/nih.aspx
dcterms.rightsHolderLippincott Williams & Wilkins
unesp.author.lattes2901888624506535
unesp.author.orcid0000-0001-7696-8219[8]
unesp.author.orcid0000-0003-4329-710X[1]
unesp.author.orcid0000-0001-6776-1515[2]
unesp.author.orcid0000-0002-7363-8211[3]

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